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A random Abstract
Our Project
Our Team
Effective Control of Salmonella Infections by Employing Combinations of Recombinant Antimicrobial Human β-Defensins hBD-1 and hBD-2.
[typhoid]
We
successfully
produced
two
human
β-defensins
(
hBD-
1
and
hBD-
2
)
in
bacteria
as
functional
peptides
and
tested
their
antibacterial
activities
against
Salmonella
enterica
serovar
Typhi
,
Escherichia
coli
,
and
Staphylococcus
aureus
employing
both
spectroscopic
and
viable
CFU
count
methods
.
Purified
peptides
showed
approximately
50
%
inhibition
of
the
bacterial
population
when
used
individually
and
up
to
90
%
when
used
in
combination
.
The
50
%
lethal
doses
(
LD
50
)
of
hBD-
1
against
S
.
Typhi
,
E
.
coli
,
and
S
.
aureus
were
0
.
36
,
0
.
40
,
and
0
.
69
μg
/
μl
,
respectively
,
while
those
for
hBD-
2
against
the
same
bacteria
were
0
.
38
,
0
.
36
,
and
0
.
66
μg
/
μl
,
respectively
.
Moreover
,
we
observed
that
bacterium-derived
antimicrobial
peptides
were
also
effective
in
increasing
survival
time
and
decreasing
bacterial
loads
in
the
peritoneal
fluid
,
liver
,
and
spleen
of
a
mouse
intraperitoneally
infected
with
S
.
Typhi
.
The
1
:
1
hBD-
1
/
hBD-
2
combination
showed
maximum
effectiveness
in
challenging
the
Salmonella
infection
in
vitro
and
in
vivo
.
We
also
observed
less
tissue
damage
and
sepsis
formation
in
the
livers
of
infected
mice
after
treatment
with
hBD-
1
and
hBD-
2
peptides
individually
or
in
combination
.
Based
on
these
findings
,
we
conclude
that
bacterium-derived
recombinant
β-defensins
(
hBD-
1
and
hBD-
2
)
are
promising
antimicrobial
peptide
(
AMP
)
-
based
substances
for
the
development
of
new
therapeutics
against
typhoid
fever
.
Diseases
Validation
Diseases presenting
"fever"
symptom
22q11.2 deletion syndrome
acute rheumatic fever
alexander disease
allergic bronchopulmonary aspergillosis
canavan disease
carcinoma of the gallbladder
child syndrome
congenital toxoplasmosis
cushing syndrome
cystinuria
dracunculiasis
erdheim-chester disease
esophageal adenocarcinoma
esophageal carcinoma
familial mediterranean fever
focal myositis
hodgkin lymphoma, classical
lamellar ichthyosis
legionellosis
locked-in syndrome
malignant atrophic papulosis
neonatal adrenoleukodystrophy
neuralgic amyotrophy
oculocutaneous albinism
papillon-lefèvre syndrome
pyomyositis
pyruvate dehydrogenase deficiency
scrub typhus
severe combined immunodeficiency
sneddon syndrome
systemic capillary leak syndrome
triple a syndrome
typhoid
waldenström macroglobulinemia
wolf-hirschhorn syndrome
This symptom has already been validated