Rare Diseases Symptoms Automatic Extraction
Home
A random Abstract
Our Project
Our Team
Magnetic Resonance Tracking of Endothelial Progenitor Cells Labeled with Alkyl-Polyethylenimine 2 kDa/Superparamagnetic Iron Oxide in a Mouse Lung Carcinoma Xenograft Model.
[severe combined immunodeficiency]
AbstractThe
potential
of
using
endothelial
progenitor
cells
(
EPCs
)
in
novel
anticancer
therapy
and
the
repair
of
vascular
injury
has
been
increasingly
recognized
.
In
the
present
study
,
EPCs
were
labeled
with
N-
alkyl-polyethylenimine
2
kDa
(
PEI
2
k
)
-
stabilized
superparamagnetic
iron
oxide
(
SPIO
)
to
facilitate
magnetic
resonance
imaging
(
MRI
)
of
EPCs
in
a
mouse
lung
carcinoma
xenograft
model
.
EPCs
derived
from
human
peripheral
blood
were
labeled
with
alkyl-
PEI
2
k
/
SPIO
.
The
viability
and
activity
of
labeled
cells
were
evaluated
using
proliferation
,
migration
,
and
tubulogenesis
assays
.
Alkyl-
PEI
2
k
/
SPIO-labeled
EPCs
were
injected
intravenously
(
group
1
)
or
mixed
and
injected
together
with
A
549
cells
subcutaneously
(
group
2
)
into
groups
of
six
mice
with
severe
combined
immunodeficiency
.
The
labeling
efficiency
with
alkyl-
PEI
2
k
/
SPIO
at
7
μg
Fe
/
mL
concentration
was
approximately
100
%
.
Quantitative
analysis
of
cellular
iron
was
6
.
062
±
0
.
050
pg
/
cell
.
No
significant
effects
on
EPC
proliferation
,
migration
,
or
tubulogenesis
were
seen
after
labeling
.
Seven
-tesla
micro-
MRI
showed
the
presence
of
schistic
or
linear
hypointense
regions
at
the
tumor
margins
starting
from
days
7
to
8
after
EPC
administration
.
This
gradually
extended
into
the
inner
tumor
layers
in
group
1
.
In
group
2
,
tumor
growth
was
accompanied
by
dispersion
of
low
-signal
intensity
regions
inside
the
tumor
.
Iron
-
positive
cells
identified
by
Prussian
blue
dye
were
seen
at
the
sites
identified
using
MRI
.
Human
CD
31
-
positive
cells
and
mouse
CD
31
-
positive
cells
were
present
in
both
groups
.
Labeling
EPCs
with
alkyl-
PEI
2
k
/
SPIO
allows
noninvasive
magnetic
resonance
investigation
of
EPC
involvement
in
tumor
neovasculature
and
is
associated
with
excellent
biocompatibility
and
MRI
sensitivity
.
Diseases
Validation
Diseases presenting
"stabilized superparamagnetic iron oxide"
symptom
severe combined immunodeficiency
You can validate or delete this automatically detected symptom
Validate the Symptom
Delete the Symptom