Differentiation-inducing and anti-proliferative activities of lupeol on canine melanoma cells.

[severe combined immunodeficiency]

Canine melanoma is the most common oral malignant tumor reported in the field of veterinary medicine . We found that lupeol , a lupine triterpene , inhibited mouse melanoma cell growth in vitro and in vivo by inducing cell differentiation . In the present study , we examined the differentiation-inducing activities of lupeol on 4 canine melanoma cells in vitro and in vivo . The induction of canine melanoma cell differentiation by lupeol was confirmed by evaluating some differentiation markers such as tyrosinase with real-time RT-PCR . Furthermore , we transplanted canine melanoma cells into a severe combined immunodeficiency mouse , and studied the anti-progressive effects of lupeol on tumor tissue . The gene expression of microphthalmia-associated transcription factor , tyrosinase , and tyrosinase -related protein-2 , which are markers of pigment cell differentiation , was induced in 4 canine oral malignant melanoma cells by lupeol , and the agent markedly inhibited tumor progression in canine melanoma -bearing mice .

Diseases
Validation

Diseases presenting "melanoma" symptom

achondroplasia

carcinoma of the gallbladder

cowden syndrome

cutaneous mastocytosis

cystinuria

dystrophic epidermolysis bullosa

epidermolysis bullosa simplex

erdheim-chester disease

erythropoietic protoporphyria

esophageal adenocarcinoma

esophageal squamous cell carcinoma

hirschsprung disease

hodgkin lymphoma, classical

inclusion body myositis

lamellar ichthyosis

liposarcoma

lymphangioleiomyomatosis

oculocutaneous albinism

papillon-lefèvre syndrome

pleomorphic liposarcoma

proteus syndrome

severe combined immunodeficiency

werner syndrome

wiskott-aldrich syndrome

This symptom has already been validated