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Efficacy of anti-CD47 antibody-mediated phagocytosis with macrophages against primary effusion lymphoma.
[primary effusion lymphoma]
Recently
,
the
critical
role
of
CD
47
on
the
surface
of
resistant
cancer
cells
has
been
proposed
in
their
evasion
of
immunosurveillance
.
Primary
effusion
lymphoma
(
PEL
)
is
a
subtype
of
aggressive
non-
Hodgkin
lymphoma
that
shows
serous
lymphomatous
effusion
in
body
cavities
,
especially
in
advanced
acquired
immunodeficiency
syndrome
(
AIDS
)
.
PEL
is
resistant
to
conventional
chemotherapy
and
has
a
poor
prognosis
.
In
this
study
,
we
evaluated
the
effect
of
anti-
CD
47
antibody
(
Ab
)
on
PEL
in
vitro
and
in
vivo
.
Surface
CD
47
of
PEL
cell
lines
was
examined
by
flow
cytometry
.
Efficacy
of
knocking
down
CD
47
or
anti-
CD
47
Ab
-mediated
phagocytosis
against
PEL
was
evaluated
using
mouse
peritoneal
macrophages
and
human
macrophages
in
vitro
.
Primary
PEL
cells
were
injected
intraperitoneally
into
NOD
/
Rag
-
2
/
Jak
3
double
-
deficient
(
NRJ
)
mice
to
establish
a
direct
xenograft
mouse
model
.
Surface
CD
47
of
PEL
cell
lines
was
highly
expressed
.
Knocking
down
CD
47
and
anti-
CD
47
Ab
promoted
phagocytic
activities
of
macrophages
in
a
CD
47
expression-dependent
manner
in
vitro
.
Treatment
with
anti-
CD
47
Ab
inhibited
ascite
formation
and
organ
invasion
completely
in
vivo
compared
with
control
IgG-treated
mice
.
CD
47
plays
the
pivotal
role
in
the
immune
evasion
of
PEL
cells
in
body
cavities
.
Therapeutic
antibody
targeting
of
CD
47
could
be
an
effective
therapy
for
PEL
.
