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Analysis of CHOP rearrangement in pleomorphic liposarcomas using fluorescence in situ hybridization.
[pleomorphic liposarcoma]
Pleomorphic
liposarcoma
(
PLS
)
is
an
aggressive
subtype
of
liposarcoma
composed
of
high
-grade
sarcoma
with
pleomorphic
lipoblasts
.
PLS
usually
exhibits
a
heterogeneous
histology
and
sometimes
has
a
myxoid
or
round
cell
area
similar
to
myxoid
/
round
cell
liposarcomas
(
MLS
/
RCs
)
.
Using
fluorescence
in
situ
hybridization
(
FISH
)
analysis
,
we
investigated
the
existence
of
CHOP
split
signals
in
various
histological
areas
of
PLS
including
the
MLS
/
RC-like
feature
and
also
estimated
the
distribution
of
various
signals
with
polyploidy
and
amplification
.
Moreover
,
to
detect
CHOP
fusion
transcripts
we
performed
nested
reverse
transcription-polymerase
chain
reaction
(
RT-PCR
)
.
Seven
PLSs
and
three
MLS
/
RCs
were
selected
for
FISH
analysis
using
the
locus-
specific
indicator
CHOP
(
12
q
13
)
dual
color
,
break
apart
probe
(
Vysis
,
USA
)
.
The
FISH
analysis
was
applied
to
formalin-fixed
,
paraffin-embedded
tissue
sections
of
representative
areas
in
all
cases
.
Six
of
seven
PLS
cases
showed
the
CHOP
split
signal
ranging
from
0
.
5
%
to
3
%
of
counted
nuclei
,
while
all
cases
of
MLS
/
RC
exhibited
CHOP
rearrangement
in
more
than
50
%
of
counted
nuclei
.
All
cases
of
PLS
showed
a
varied
distribution
of
extra
signals
with
polyploidy
and
amplification
in
each
histological
area
.
No
CHOP
fusion
transcript
was
found
in
any
case
of
PLS
by
nested
RT-PCR
.
A
CHOP
rearrangement
in
PLS
should
be
recognized
only
as
a
representative
part
of
complex
karyotypes
,
because
the
number
of
cells
with
split
signals
was
minute
compared
with
that
of
MLS
/
RC
,
and
the
signals
were
found
in
any
area
despite
their
histological
differences
.
The
cytogenetic
background
of
PLS
and
that
of
MLS
/
RC
are
obviously
different
despite
histological
similarity
.
