Diagnostic Value of MDM2 and DDIT3 Fluorescence In Situ Hybridization in Liposarcoma Classification: A Single-Institution Experience.

[pleomorphic liposarcoma]

The amplification of murine double minutes (MDM 2 ) is the primary feature of well-differentiated liposarcomas (WDLPS ) and dedifferentiated liposarcomas (DDLPS ), while DDIT 3 rearrangement is the main one of myxoid liposarcomas (MLPS ). Our aim was to evaluate the added value of MDM 2 amplification and DDIT 3 rearrangement in making a diagnosis and classifying lipogenic tumors .Eighty -two cases of liposarcoma and 60 lipomas diagnosed between 1995 and 2010 were analysed for MDM 2 amplification and DDIT 3 rearrangement using a fluorescence in situ hybridization (FISH ). The subtypes of liposarcoma were reclassified according to the molecular results , whose results were reviewed with an analysis of the relevant histologic and immunohistochemical findings .One case of lipoma (1 .67 %) was reclassified as a WDLPS . Of the liposarcomas , 13 .4 % (16 /82 ) were reclassified after the molecular testing . Five cases of MLPS were reclassified as four cases of DDLPS and one case of myxoid lipoma . Two cases of WDLPS were reclassified as one case of spindle cell lipoma and another case of myxofibrosarcoma . Four cases of DDLPS were reclassified as two cases of leiomyosarcoma , one case of angiomyolipoma and another case of fibroinflammatory lesion . Of the six cases of pleomorphic liposarcoma , five were reclassified as DDLPS .In our series , a critical revision of diagnosis was found at a rate of 3 .5 % (5 /142 ) after a review of the lipomatous lesions . The uses of molecular testing by MDM 2 and DDIT 3 FISH were valuable to make an accurate subtyping of liposarcomas as well as to differentiate WDLPS from benign lipomatous tumor .

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Diseases presenting "ddit3 rearrangement in making a diagnosis and classifying lipogenic tumors" symptom

pleomorphic liposarcoma

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