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The co-inheritance of alpha-thalassemia and sickle cell anemia is associated with better hematological indices and lower consultations rate in Cameroonian patients and could improve their survival.
[alpha-thalassemia]
Co
-inheritance
of
α-thalassemia
was
reported
to
be
associated
with
a
delayed
age
of
disease
onset
among
Cameroonian
Sickle
Cell
Anemia
(
SCA
)
patients
.
The
present
study
aimed
to
explore
the
correlation
between
α-thalassemia
,
hematological
indices
,
and
clinical
events
in
these
patients
.
We
studied
161
Cameroonian
SCA
patients
and
103
controls
(
59
.
1
%
HbAA
)
with
median
ages
of
17
.
5
and
23
years
.
RFLP-PCR
was
used
to
confirm
SCA
genotype
and
to
describe
haplotypes
in
the
HBB
-like
genes
cluster
.
Multiplex
Gap
-
PCR
was
performed
to
investigate
the
3
.
7
kb
α-globin
gene
deletions
.
SNaPshot
PCR
,
capillary
electrophoresis
and
cycle
sequencing
were
used
for
the
genotyping
of
10
SNPs
in
BCL
11
A
,
HMIP
1
/
2
,
OR
51
B
5
/
6
and
HBG
loci
,
known
to
influence
HbF
levels
.
Generalised
linear
regression
models
adjusted
for
age
,
sex
and
SNPs
genotypes
was
used
to
investigate
effects
of
α-thalassemia
on
clinical
and
hematological
indices
.
The
median
rate
of
vaso-occlusive
painful
crisis
and
hospitalisations
was
two
and
one
per
year
,
respectively
.
Stroke
was
reported
in
eight
cases
(
7
.
4
%
)
.
Benin
haplotype
was
the
most
prevalent
(
66
.
3
%
;
n
 
=
 
208
chromosomes
)
.
Among
patients
,
37
.
3
%
(
n
 
=
 
60
)
had
at
least
one
3
.
7
kb
deletion
,
compared
to
10
.
9
%
(
n
 
=
 
6
)
among
HbAA
controls
(
p
<
0
.
001
)
.
Among
patients
,
the
median
RBC
count
increased
with
the
number
of
3
.
7
kb
deletions
[
2
.
6
,
3
.
0
and
3
.
4
million
/
dl
,
with
no
,
one
and
two
deletions
(
p
 
=
 
0
.
01
)
]
.
The
median
MCV
decreased
with
the
number
of
3
.
7
kb
deletion
[
86
,
80
,
and
68
fl
,
with
no
,
one
and
two
deletions
(
p
<
0
.
0001
)
]
,
as
well
as
median
WBC
counts
[
13
.
2
,
10
.
5
and
9
.
8
×
109
/
L
(
p
<
0
.
0001
.
The
co
-inheritance
of
α-thalassemia
was
associated
with
lower
consultations
rate
(
p
 
=
 
0
.
038
)
.
The
co
-inheritance
of
α-thalassemia
and
SCA
is
associated
with
improved
hematological
indices
,
and
lower
consultations
rate
in
this
group
of
patients
.
This
could
possibly
improve
their
survival
and
explain
the
higher
proportion
of
α-thalassemia
among
patients
than
controls
.
Diseases
Validation
Diseases presenting
"hospitalisations was two and one per year"
symptom
alpha-thalassemia
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