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Panax notoginseng saponins inhibit areca nut extract-induced oral submucous fibrosis in vitro.
[oral submucous fibrosis]
Oral
submucous
fibrosis
(
OSF
)
is
a
premalignant
and
fibrosing
disease
,
which
is
closely
associated
with
the
habit
of
chewing
areca
nut
.
Panax
notoginseng
Buck
F
.
H
.
Chen
is
an
often
used
antifibrotic
and
antitumor
agent
.
To
treat
areca
nut-induced
OSF
,
we
have
developed
a
chewable
tablet
,
in
which
one
of
the
major
medicines
is
total
Panax
notoginseng
saponins
(
PNS
)
.
In
this
study
,
we
have
investigated
the
antifibrotic
effect
and
mechanism
of
PNS
on
areca
nut-induced
OSF
in
vitro
.
Through
human
procollagen
gene
promoter
luciferase
reporter
plasmid
,
hydroxyproline
assay
,
gelatin
zymography
,
qRT-PCR
,
ELISA
,
and
Western
blot
,
the
influences
of
PNS
on
areca
nut
extract
(
ANE
)
-
induced
cell
growth
,
collagen
accumulation
,
procollagen
gene
transcription
,
MMP-
2
/
-
9
activity
,
MMP-
1
/
-
13
and
TIMP-
1
/
-
2
expression
,
cytokine
secretion
,
and
the
activation
of
PI
3
K
/
AKT
,
ERK
/
JNK
/
p
38
MAPK
,
and
TGF
β
/
Smads
pathways
were
detected
.
Panax
notoginseng
saponins
could
inhibit
the
ANE-induced
abnormal
growth
and
collagen
accumulation
of
oral
mucosal
fibroblasts
in
a
concentration-dependent
manner
.
PNS
(
25
μg
/
ml
)
could
significantly
inhibit
the
ANE-induced
expression
of
Col
1
A
1
and
Col
3
A
1
,
augment
the
ANE-induced
decrease
of
MMP-
2
/
-
9
activity
,
inhibit
the
ANE-induced
increase
of
TIMP-
1
/
-
2
expression
,
and
decrease
the
ANE-induced
transcription
and
release
of
CTGF
,
TGFβ
1
,
IL
-
6
,
and
TNF
α
.
PNS
(
25
μg
/
ml
)
also
significantly
inhibited
the
ANE-induced
activation
of
AKT
and
ERK
/
JNK
/
p
38
MAPK
pathways
in
oral
mucosal
fibroblasts
and
the
ANE-induced
activation
of
TGF
β
/
smad
pathway
in
HaCaT
cells
.
P
anax
notoginseng
saponins
possess
excellent
anti-
OSF
activity
,
and
its
mechanism
may
be
related
to
its
ability
to
inhibit
the
ANE-induced
activation
of
PI
3
K
/
AKT
,
ERK
/
JNK
/
p
38
MAPK
,
and
TGFβ
/
smad
pathways
.
Diseases
Validation
Diseases presenting
"abnormal growth"
symptom
cutaneous mastocytosis
oral submucous fibrosis
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