Controlled human malaria infection of Tanzanians by intradermal injection of aseptic, purified, cryopreserved Plasmodium falciparum sporozoites.

[alpha-thalassemia]

Controlled human malaria infection (CHMI ) by mosquito bite has been used to assess anti-malaria interventions in > 1 ,500 volunteers since development of methods for infecting mosquitoes by feeding on Plasmodium falciparum (Pf ) gametocyte cultures . Such CHMIs have never been used in Africa . Aseptic , purified , cryopreserved Pf sporozoites , PfSPZ Challenge , were used to infect Dutch volunteers by intradermal injection . We conducted a double -blind , placebo-controlled trial to assess safety and infectivity of PfSPZ Challenge in adult male Tanzanians . Volunteers were injected intradermally with 10 ,000 (N = 12 ) or 25 ,000 (N = 12 ) PfSPZ or normal saline (N = 6 ). PfSPZ Challenge was well tolerated and safe . Eleven of 12 and 10 of 11 subjects , who received 10 ,000 and 25 ,000 PfSPZ respectively , developed parasitemia . In 10 ,000 versus 25 ,000 PfSPZ groups geometric mean days from injection to Pf positivity by thick blood film was 15 .4 versus 13 .5 (P = 0 .023 ). Alpha-thalassemia heterozygosity had no apparent effect on infectivity . PfSPZ Challenge was safe , well tolerated , and infectious .

Diseases
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Diseases presenting "thick blood film" symptom

alpha-thalassemia

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