Rare Diseases Symptoms Automatic Extraction
Home
A random Abstract
Our Project
Our Team
Association between mitochondrial C-tract alteration and tobacco exposure in oral precancer cases.
[oral submucous fibrosis]
Tobacco
exposure
is
a
known
risk
factor
for
oral
cancer
.
India
is
home
to
oral
cancer
epidemic
chiefly
due
to
the
prevalent
use
of
both
smoke
and
smokeless
tobacco
.
To
reduce
the
related
morbidity
early
detection
is
required
.
The
key
to
this
is
detailing
molecular
events
during
early
precancer
stage
.
Mitochondrion
is
an
important
cellular
organelle
involved
in
cell
metabolism
and
apoptosis
.
Mitochondrial
dysfunction
is
thought
to
be
the
key
event
in
oncogenesis
.
Last
decade
has
seen
a
spurt
of
reports
implicating
mitochondrial
mutations
in
oral
carcinogenesis
.
However
,
there
are
few
reports
that
study
mitochondrial
deoxyribonucleic
acid
(
mtDNA
)
changes
in
oral
precancer
.
This
study
aims
to
understand
and
link
effect
of
tobacco
exposure
on
mtDNA
in
oral
precancer
cases
.
A
total
of
100
oral
precancer
cases
of
which
50
oral
leukoplakia
and
50
oral
submucous
fibrosis
were
recruited
in
the
study
and
a
detailed
questionnaire
were
filled
about
the
tobacco
habits
.
Their
tissue
and
blood
samples
were
collected
.
Total
genomic
DNA
was
isolated
from
both
sources
.
Mitochondrial
C-
tract
was
amplified
and
bidirectional
sequencing
was
carried
out
.
Mutations
were
scored
over
matched
blood
DNA
.
There
was
a
significant
association
between
the
presence
of
mitochondrial
C-
tract
alteration
and
duration
of
tobacco
exposure
.
The
probability
increased
with
increasing
duration
of
tobacco
consumption
.
The
risk
of
having
this
alteration
was
more
in
chewers
than
in
smokers
.
Tobacco
in
both
form
,
chewable
and
smoke
,
is
oncogenic
and
causes
early
changes
in
mitochondrial
genome
and
chances
increases
with
increasing
duration
of
tobacco
consumption
.