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Comparative evaluation of genotoxicity by micronucleus assay in the buccal mucosa over comet assay in peripheral blood in oral precancer and cancer patients.
[oral submucous fibrosis]
Early
detection
and
quantification
of
DNA
damage
in
oral
premalignancy
or
malignancy
may
help
in
management
of
the
disease
and
improve
survival
rates
.
The
comet
assay
has
been
successfully
utilised
to
detect
DNA
damage
in
oral
premalignant
or
malignancy
.
However
,
due
to
the
invasive
nature
of
collecting
blood
,
it
may
be
painful
for
many
unwilling
patients
.
This
study
compares
the
micronucleus
(
MN
)
assay
in
oral
buccal
mucosa
cells
with
the
comet
assay
in
peripheral
blood
cells
in
a
subset
of
oral
habit-induced
precancer
and
cancer
patients
.
For
this
,
MN
assay
of
exfoliated
epithelial
cells
was
compared
with
comet
assay
of
peripheral
blood
leucocytes
among
260
participants
,
including
those
with
oral
lichen
planus
(
OLP
;
n
=
52
)
,
leukoplakia
(
LPK
;
n
=
51
)
,
oral
submucous
fibrosis
(
OSF
;
n
=
51
)
,
oral
squamous
cell
carcinoma
(
OSCC
;
n
=
54
)
and
normal
volunteers
(
n
=
52
)
.
Among
the
precancer
groups
,
LPK
patients
showed
significantly
higher
levels
of
DNA
damage
as
reflected
by
both
comet
tail
length
(
P
<
0
.
0001
)
and
micronuclei
(
MNi
)
frequency
(
P
=
0
.
0009
)
.
The
DNA
damage
pattern
in
precancer
and
cancer
patients
was
OLP
<
OSF
<
LPK
<
OSCC
,
and
with
respective
oral
habits
,
it
was
multiple
habits
>
cigarette
+
khaini
>
cigarette
smokers
>
areca
+
khaini
>
areca
.
There
was
no
significant
difference
in
the
comet
length
and
MNi
frequency
between
males
and
females
who
had
oral
chewing
habits
.
An
overall
significant
correlation
was
observed
between
MNi
frequency
and
comet
tail
length
with
r
=
0
.
844
and
P
<
0
.
0001
.
Thus
,
the
extent
of
DNA
damage
evaluation
by
the
comet
assay
in
peripheral
blood
cells
is
perfectly
reflected
by
the
MN
assay
on
oral
exfoliated
epithelial
cells
,
and
MNi
frequency
can
be
used
with
the
same
effectiveness
and
greater
efficiency
in
early
detection
of
oral
premalignant
conditions
.
Diseases
Validation
Diseases presenting
"squamous cell carcinoma"
symptom
carcinoma of the gallbladder
child syndrome
dystrophic epidermolysis bullosa
epidermolysis bullosa simplex
esophageal adenocarcinoma
esophageal carcinoma
esophageal squamous cell carcinoma
junctional epidermolysis bullosa
kallmann syndrome
kindler syndrome
liposarcoma
monosomy 21
oculocutaneous albinism
oral submucous fibrosis
papillon-lefèvre syndrome
This symptom has already been validated