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Comparative evaluation of genotoxicity by micronucleus assay in the buccal mucosa over comet assay in peripheral blood in oral precancer and cancer patients.
[oral submucous fibrosis]
Early
detection
and
quantification
of
DNA
damage
in
oral
premalignancy
or
malignancy
may
help
in
management
of
the
disease
and
improve
survival
rates
.
The
comet
assay
has
been
successfully
utilised
to
detect
DNA
damage
in
oral
premalignant
or
malignancy
.
However
,
due
to
the
invasive
nature
of
collecting
blood
,
it
may
be
painful
for
many
unwilling
patients
.
This
study
compares
the
micronucleus
(
MN
)
assay
in
oral
buccal
mucosa
cells
with
the
comet
assay
in
peripheral
blood
cells
in
a
subset
of
oral
habit-induced
precancer
and
cancer
patients
.
For
this
,
MN
assay
of
exfoliated
epithelial
cells
was
compared
with
comet
assay
of
peripheral
blood
leucocytes
among
260
participants
,
including
those
with
oral
lichen
planus
(
OLP
;
n
=
52
)
,
leukoplakia
(
LPK
;
n
=
51
)
,
oral
submucous
fibrosis
(
OSF
;
n
=
51
)
,
oral
squamous
cell
carcinoma
(
OSCC
;
n
=
54
)
and
normal
volunteers
(
n
=
52
)
.
Among
the
precancer
groups
,
LPK
patients
showed
significantly
higher
levels
of
DNA
damage
as
reflected
by
both
comet
tail
length
(
P
<
0
.
0001
)
and
micronuclei
(
MNi
)
frequency
(
P
=
0
.
0009
)
.
The
DNA
damage
pattern
in
precancer
and
cancer
patients
was
OLP
<
OSF
<
LPK
<
OSCC
,
and
with
respective
oral
habits
,
it
was
multiple
habits
>
cigarette
+
khaini
>
cigarette
smokers
>
areca
+
khaini
>
areca
.
There
was
no
significant
difference
in
the
comet
length
and
MNi
frequency
between
males
and
females
who
had
oral
chewing
habits
.
An
overall
significant
correlation
was
observed
between
MNi
frequency
and
comet
tail
length
with
r
=
0
.
844
and
P
<
0
.
0001
.
Thus
,
the
extent
of
DNA
damage
evaluation
by
the
comet
assay
in
peripheral
blood
cells
is
perfectly
reflected
by
the
MN
assay
on
oral
exfoliated
epithelial
cells
,
and
MNi
frequency
can
be
used
with
the
same
effectiveness
and
greater
efficiency
in
early
detection
of
oral
premalignant
conditions
.
Diseases
Validation
Diseases presenting
"oral premalignant"
symptom
oral submucous fibrosis
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