Genotyping of α-thalassemias by the colorimetric nanogold probes.

[alpha-thalassemia]

The novel colorimetric nanogold probe was created to genotype subgroups of the mostly found α-thalassemias . They are α-thalassemia 1 (SEA and THAI deletion ) and α-thalassemia 2 (3 .7 -kb and 4 .2 -kb deletion ).The genotyping was performed by two -steps hybridizations . First step was hybridization of target DNA with the nanogold mixed probes of either α-thalassemia 1 or α-thalassemia 2 . No hybridization in both reactions showing blue color indicated absence of abnormal genes causing these α-thalassemias . Positive reaction showing either red or purple color was further analyzed in second hybridization with the nanogold single probe . Positive of α-thalassemia 1 was genotyped with the single probes of both SEA and THAI deletion while those of α-thalassemia 2 were genotyped with both 3 .7 -kb and 4 .2 -kb deletion .Genotypic potency of the nanogold mixed and single probes was evaluated using both known diagnosed and suspected clinical samples . The results by naked eye were consistence with those analyzed by standard agarose gel electrophoresis .Potency of the colorimetric nanogold α-thalassemia probes was accurate , precise , sensitive , specific , simple , cheap and field applicable . Color reaction was simply visualized by naked eye . This development is an example of colorimetric molecular diagnosis which can be applied in any genetic detection .

Diseases
Validation

Diseases presenting "single probes of both sea" symptom

alpha-thalassemia

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