Expression and location of phospho-Artemis (Serine516) in hair follicles during induced growth of mouse hair.

[omenn syndrome]

Artemis has been implicated in having a role in NHEJ , and it is also a multifunctional protein . Previous studies have found Omenn syndrome -like phenotype due to Artemis mutations and associated with alopecia . As Artemis phosphorylation in its c-terminus including Serine 516 is prerequisite for the Artemis endonuclease reaction , we postulate that Artemis (Serine 516 ) may be expressed in hair follicle and relate to hair cycling . In this study , hair growth in C 5 7 BL /6 mice was induced by plucking the telogen hair on the back . Expression of Artemis (Serine 516 ) in hair follicles during the hair growth cycle was evaluated by immunofluorescence using cryosections and a specific polyclonal anti-Artemis (Serine 516 ) immunoglobulin G (IgG ) antibody . It was detected in germ cells , cap , and club hair adjoining the epidermis in telogen . In anagen II , intense staining for Artemis (Serine 516 ) was found in the whole interfollicular epidermis , and in strand keratinocytes . In anagen IV , intense staining for Artemis (Serine 516 ) was detected in basal cells and upper of outer root sheath (ORS ) and inner root sheath (IRS ). But only upper ORS and lower medulla were stained positive in anagen VI . Upper ORS and lower cortex were positively stained with Artemis (Serine 516 ) in catagen . Based on the phenomenon that the expression of Artemis (Serine 516 ) in mid-anagen and mature anagen was stronger than that in telogen and catagen , we suggest it may take roles in induced growth of mouse hair .