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A random Abstract
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Biallelic genome modification in F(0) Xenopus tropicalis embryos using the CRISPR/Cas system.
[oculocutaneous albinism]
Gene
inactivation
is
an
important
tool
for
correlation
of
phenotypic
and
genomic
data
,
allowing
researchers
to
infer
normal
gene
function
based
on
the
phenotype
when
the
gene
is
impaired
.
New
and
better
approaches
are
needed
to
overcome
the
shortfalls
of
existing
methods
for
any
significant
acceleration
of
scientific
progress
.
We
have
adapted
the
CRISPR
/
Cas
system
for
use
in
Xenopus
tropicalis
and
report
on
the
efficient
creation
of
mutations
in
the
gene
encoding
the
enzyme
tyrosinase
,
which
is
responsible
for
oculocutaneous
albinism
.
Biallelic
mutation
of
this
gene
was
detected
in
the
F
0
generation
,
suggesting
targeting
efficiencies
similar
to
that
of
TALENs
.
We
also
find
that
off-target
mutagenesis
seems
to
be
negligible
,
and
therefore
,
CRISPR
/
Cas
may
be
a
useful
system
for
creating
genome
modifications
in
this
important
model
organism
.