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A random Abstract
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Interstitial cells of Cajal in the human normal urinary bladder and in the bladder of patients with megacystis-microcolon intestinal hypoperistalsis syndrome.
[megacystis-microcolon-intestinal hypoperistalsis syndrome]
To
investigate
the
distribution
of
c-kit-
positive
interstitial
cells
of
Cajal
(
ICCs
)
in
normal
bladder
and
bladders
from
patients
with
megacystis
-
microcolon
-
intestinal
peristalsis
syndrome
(
MMIHS
,
a
rare
congenital
and
generally
fatal
cause
of
functional
intestinal
obstruction
in
the
newborn
)
,
the
most
characteristic
feature
of
which
is
abdominal
distension
caused
by
a
distended
unobstructed
urinary
bladder
.
Full-thickness
bladder
specimens
were
obtained
from
four
infants
with
MMIHS
and
four
controls
,
and
processed
as
paraffin-wax
and
frozen
sections
.
Sections
were
assessed
using
single
immunohistochemistry
with
monoclonal
and
polyclonal
anti-c-kit
antibodies
.
Anti-alpha-smooth
muscle
actin
(
SMA
)
antibody
was
used
to
investigate
the
contractile
apparatus
in
smooth
muscle
cells
of
the
urinary
bladder
.
Specimens
were
examined
using
light
and
confocal
scanning
microscopy
.
There
were
many
c-kit
positive
ICCs
in
the
normal
urinary
bladder
,
appearing
as
small
,
long
,
bipolar
cells
with
only
two
long
and
several
short
processes
.
In
contrast
,
ICCs
were
absent
in
the
MMIHS
bladder
.
alpha-
SMA
immunoreactivity
was
lower
in
MMIHS
urinary
bladder
than
in
control
sections
.
This
study
shows
for
the
first
time
the
presence
of
c-kit-
positive
ICCs
in
the
normal
human
urinary
bladder
.
The
lack
of
ICCs
in
the
MMIHS
bladder
may
contribute
to
the
voiding
dysfunction
in
this
disease
.