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Application of three-dimensional imaging to the intestinal crypt organoids and biopsied intestinal tissues.
[megacystis-microcolon-intestinal hypoperistalsis syndrome]
Two
-dimensional
(
2
D
)
histopathology
is
the
standard
analytical
method
for
intestinal
biopsied
tissues
;
however
,
the
role
of
3
-
dimensional
(
3
D
)
imaging
system
in
the
analysis
of
the
intestinal
tissues
is
unclear
.
The
3
D
structure
of
the
crypt
organoids
from
the
intestinal
stem
cell
culture
and
intestinal
tissues
from
the
donors
and
recipients
after
intestinal
transplantation
was
observed
using
a
3
D
imaging
system
and
compared
with
2
D
histopathology
and
immunohistochemistry
.
The
crypt
organoids
and
intestinal
tissues
showed
well-defined
3
D
structures
.
The
3
D
images
of
the
intestinal
tissues
with
acute
rejection
revealed
absence
of
villi
and
few
crypts
,
which
were
consistent
with
the
histopathological
features
.
In
the
intestinal
transplant
for
megacystis
microcolon
intestinal
hypoperistalsis
syndrome
,
the
donor
's
intestinal
tissues
had
well-developed
nerve
networks
and
interstitial
cells
of
Cajal
(
ICCs
)
in
the
muscle
layer
,
while
the
recipient
's
intestinal
tissues
had
distorted
nerve
network
and
the
ICCs
were
few
and
sparsely
distributed
,
relative
to
those
of
the
donor
.
The
3
D
images
showed
a
clear
spatial
relationship
between
the
microstructures
of
the
small
bowel
and
the
features
of
graft
rejection
.
In
conclusion
,
integration
of
the
3
D
imaging
and
2
D
histopathology
provided
a
global
view
of
the
intestinal
tissues
from
the
transplant
patients
.
