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Effect of rMnSOD on survival signaling in pediatric high risk T-cell acute lymphoblastic leukaemia.
[liposarcoma]
Manganese
superoxide
dismutase
(
MnSOD
)
is
a
mitochondrial
enzyme
that
defends
against
oxidative
damage
due
to
reactive
oxygen
species
(
ROS
)
.
A
new
isoform
of
MnSOD
with
cytotoxic
activity
was
recently
discovered
in
liposarcoma
cells
.
Here
,
we
tested
the
effectiveness
of
a
recombinant
form
of
this
isoform
(
rMnSOD
)
on
leukemic
T
cells
,
Jurkat
cells
,
and
normal
lymphocytes
.
Our
results
confirm
that
leukemic
T
cells
can
internalize
rMnSOD
and
that
rMnSOD
causes
apoptosis
of
99
%
of
leukemic
cells
without
showing
toxic
effects
on
healthy
cells
.
Using
light
and
electron
microscopy
,
we
determined
that
an
rMnSOD
concentration
of
0
.
067
μM
most
effective
on
apoptosis
induction
.
Western
blot
analysis
showed
that
treatment
with
0
.
067
μM
rMnSOD
resulted
in
high
expression
of
the
pro-apoptotic
protein
Bax
and
low
expression
of
the
anti-apoptotic
protein
Bcl-
2
in
leukemia
cells
.
Concerning
signal
transduction
pathway
no
influence
was
observed
after
treatment
except
for
Jurkat
cells
showing
a
slightly
decreased
expression
of
ERK
phosphorylation
.
These
results
suggest
that
rMnSOD
may
be
an
effective
and
non-toxic
treatment
option
for
T
-
cell
leukemia
.
J
.
Cell
.
Physiol
.
©
2014
Wiley
Periodicals
,
Inc
.
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"slightly decreased expression"
symptom
liposarcoma
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