Rare Diseases Symptoms Automatic Extraction
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A random Abstract
Our Project
Our Team
Analysis of Legionella infection using RNAi in Drosophila cells.
[legionellosis]
RNA
interference
(
RNAi
)
is
the
process
of
specific
gene
silencing
by
the
use
of
double
-stranded
RNA
(
dsRNA
)
.
In
cultured
Drosophila
cells
,
RNAi
methodologies
are
well
established
and
easily
executed
:
dsRNA
,
when
added
to
the
cell
culture
medium
,
is
efficiently
internalized
by
the
cells
and
,
through
the
activity
of
endogenous
processing
machinery
,
targets
the
specified
mRNA
for
degradation
resulting
in
reduced
levels
of
its
encoded
protein
.
This
technique
has
proven
very
useful
in
studying
the
role
of
host
genes
during
Legionella
pneumophila
infections
,
as
it
allows
the
effect
of
host
factor
depletion
on
intracellular
growth
of
the
bacterium
to
be
examined
.
In
this
chapter
we
present
the
methods
commonly
used
in
our
laboratory
to
study
intracellular
growth
of
L
.
pneumophila
using
dsRNA
in
Drosophila
cells
.
