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Widespread molecular detection of Legionella pneumophila Serogroup 1 in cold water taps across the United States.
[legionellosis]
In
the
United
States
,
6
,
868
cases
of
legionellosis
were
reported
to
the
Center
for
Disease
Control
and
Prevention
in
2009
-
2010
.
Of
these
reports
,
it
is
estimated
that
84
%
are
caused
by
the
microorganism
Legionella
pneumophila
Serogroup
(
Sg
)
1
.
Legionella
spp
.
have
been
isolated
and
recovered
from
a
variety
of
natural
freshwater
environments
.
Human
exposure
to
L
.
pneumophila
Sg
1
may
occur
from
aerosolization
and
subsequent
inhalation
of
household
and
facility
water
.
In
this
study
,
two
primer
/
probe
sets
(
one
able
to
detect
L
.
pneumophila
and
the
other
L
.
pneumophila
Sg
1
)
were
determined
to
be
highly
sensitive
and
selective
for
their
respective
targets
.
Over
272
water
samples
,
collected
in
2009
and
2010
from
68
public
and
private
water
taps
across
the
United
States
,
were
analyzed
using
the
two
qPCR
assays
to
evaluate
the
incidence
of
L
.
pneumophila
Sg
1
.
Nearly
half
of
the
taps
showed
the
presence
of
L
.
pneumophila
Sg
1
in
one
sampling
event
,
and
16
%
of
taps
were
positive
in
more
than
one
sampling
event
.
This
study
is
the
first
United
States
survey
to
document
the
occurrence
and
colonization
of
L
.
pneumophila
Sg
1
in
cold
water
delivered
from
point
of
use
taps
.