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Keratinocyte ATP binding cassette transporter expression is regulated by ultraviolet light.
[lamellar ichthyosis]
Many
ATP
binding
cassette
(
ABC
)
transporters
are
important
regulators
of
lipid
homeostasis
and
have
been
implicated
in
keratinocyte
lipid
transport
.
Ultraviolet
(
UV
)
light
exposure
is
a
known
epidermal
stressor
,
which
amongst
other
effects
causes
lipid
alterations
and
defective
lamellar
body
biogenesis
.
To
elucidate
the
background
of
these
lipid
changes
we
studied
the
effect
of
UVB
light
on
ABC
transporter
expression
.
The
effect
of
UVB
treatment
on
the
levels
of
47
known
human
ABC
transporter
mRNAs
was
analyzed
in
normal
human
epidermal
keratinocytes
.
Immunoblots
and
promoter
assays
were
carried
out
for
ABCA
1
and
ABCG
1
.
The
mRNA
levels
of
cholesterol
transport
regulators
ABCA
1
and
ABCG
1
were
markedly
downregulated
by
UVB
,
parallel
to
the
lamellar
ichthyosis
related
glucosylceramide
transporter
ABCA
12
and
the
suspected
sphingosine-
1
-
phosphate
and
cholesterol
sulfate
transporter
ABCC
1
.
The
long
but
not
the
short
alternative
splice
variant
of
the
ABCF
2
was
found
to
be
markedly
upregulated
rapidly
after
UVB
irradiation
.
Immunoblot
confirmed
ABCA
1
and
ABCG
1
protein
downregulation
,
and
luciferase
assays
showed
suppression
of
their
promoters
by
UVB
.
These
proteins
mostly
transport
lipids
,
which
account
for
the
integrity
of
the
epidermal
barrier
;
therefore
our
findings
on
the
UVB
regulation
of
ABC
transporters
may
explain
the
appearance
of
barrier
dysfunction
after
UVB
exposure
.
