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Induction of α1 and α2 gene expression in selective immunoglobulin A deficiency.
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Immunoglobulin
A
deficiency
(
IgAD
)
is
the
most
common
immunodeficiency
,
but
the
pathogenesis
of
most
cases
of
IgAD
is
poorly
understood
.
The
gene
and
protein
expression
levels
of
members
of
the
IgA
subclasses
in
IgAD
patients
were
analyzed
by
a
reverse
transcriptase
(
RT
)
-
PCR
method
that
could
differentiate
between
α
1
and
α
2
gene
expression
.
Three
selective
,
5
partial
and
2
secondary
IgAD
patients
were
examined
.
Peripheral
blood
mononuclear
cells
which
were
unstimulated
or
stimulated
with
TGF-β
1
and
PMA
for
24
h
were
cultured
.
The
IgA
1
/
IgA
2
expression
ratios
were
measured
by
zone
densitometry
.
Three
bands
appeared
(
the
α
1
and
α
2
genes
and
a
hetero-duplex
formation
)
,
owing
to
the
difference
of
39
bases
between
α
1
and
α
2
mRNAs
.
In
the
controls
,
there
were
no
significant
differences
in
the
IgA
1
/
IgA
2
ratios
between
unstimulated
and
stimulated
cells
.
In
selective
IgAD
patients
,
both
α
1
and
α
2
gene
expression
was
induced
following
stimulation
,
and
α
1
gene
expression
was
induced
more
dominantly
than
in
the
other
IgAD
patients
following
stimulation
.
Based
on
our
results
,
suppression
of
α
1
gene
expression
may
be
related
to
the
pathogenesis
of
IgAD
.
