Protein changes in immunodepleted cerebrospinal fluid from a transgenic mouse model of Alexander disease detected using mass spectrometry.

[alexander disease]

Cerebrospinal fluid (CSF ) is a low protein content biological fluid with a dynamic range spanning at least 9 orders of magnitude in protein content and is in direct contact with the brain . A modified IgY-14 immunodepletion treatment was performed to enhance analysis of the low volumes of CSF that are obtainable from mice . As a model system in which to test this approach , we utilized transgenic mice that overexpress the intermediate filament glial fibrillary acidic protein (GFAP ). These mice are models for Alexander disease (AxD ), a severe leukodystrophy in humans . From the CSF of control and transgenic mice we report the identification of 289 proteins , with relative quantification of 103 proteins . Biological and technical triplicates were performed to address animal variability as well as reproducibility in mass spectrometric analysis . Relative quantitation was performed using distributive normalized spectral abundance factor (dNSAF ) spectral counting analysis . A panel of biomarker proteins with significant changes in the CSF of GFAP transgenic mice has been identified with validation from enzyme-linked immunosorbent assay (ELISA ) and microarray data , demonstrating the utility of our methodology and providing interesting targets for future investigations on the molecular and pathological aspects of AxD .

Diseases
Validation

Diseases presenting "low protein content" symptom

alexander disease

You can validate or delete this automatically detected symptom