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Astrocytic TDP-43 pathology in Alexander disease.
[alexander disease]
Alexander
disease
(
AxD
)
is
a
rare
neurodegenerative
disorder
characterized
pathologically
by
the
presence
of
eosinophilic
inclusions
known
as
Rosenthal
fibers
(
RFs
)
within
astrocytes
,
and
is
caused
by
dominant
mutations
in
the
coding
region
of
the
gene
encoding
glial
fibrillary
acidic
protein
(
GFAP
)
.
GFAP
is
the
major
astrocytic
intermediate
filament
,
and
in
AxD
patient
brain
tissue
GFAP
is
a
major
component
of
RFs
.
TAR
DNA
binding
protein
of
43
kDa
(
TDP-
43
)
is
the
major
pathological
protein
in
almost
all
cases
of
the
neurodegenerative
disease
amyotrophic
lateral
sclerosis
(
ALS
)
and
∼
50
%
of
frontotemporal
lobar
degeneration
(
FTLD
)
,
designated
as
FTLD-TDP
.
In
ALS
and
FTLD-TDP
,
TDP-
43
becomes
insoluble
,
ubiquitinated
,
and
pathologically
phosphorylated
and
accumulates
in
cytoplasmic
inclusions
in
both
neurons
and
glia
of
affected
brain
and
spinal
cord
regions
.
Previously
,
TDP-
43
was
detected
in
RFs
of
human
pilocytic
astrocytomas
;
however
,
involvement
of
TDP-
43
in
AxD
has
not
been
determined
.
Here
we
show
that
TDP-
43
is
present
in
RFs
in
AxD
patient
brains
,
and
that
insoluble
phosphorylated
full-length
and
high
molecular
weight
TDP-
43
accumulates
in
white
matter
of
such
brains
.
Phosphorylated
TDP-
43
also
accumulates
in
the
detergent-insoluble
fraction
from
affected
brain
regions
of
Gfap
(
R
236
H
/
+
)
knock-
in
mice
,
which
harbor
a
GFAP
mutation
homologous
to
one
that
causes
AxD
in
humans
,
and
TDP-
43
colocalizes
with
astrocytic
RF
pathology
in
Gfap
(
R
236
H
/
+
)
mice
and
transgenic
mice
overexpressing
human
wild-
type
GFAP
.
These
findings
suggest
common
pathogenic
mechanisms
in
ALS
,
FTLD
,
and
AxD
,
and
this
is
the
first
report
of
TDP-
43
involvement
in
a
neurological
disorder
primarily
affecting
astrocytes
.
Diseases
Validation
Diseases presenting
"brain regions"
symptom
22q11.2 deletion syndrome
alexander disease
cadasil
cushing syndrome
fabry disease
krabbe disease
pyruvate dehydrogenase deficiency
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