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The cystathionine beta-synthase variant c.844_845ins68 protects against CNS demyelination in X-linked adrenoleukodystrophy.
[adrenomyeloneuropathy]
The
clinical
course
of
X-
linked
adrenoleukodystrophy
(
X-
ALD
)
is
of
unexplained
heterogeneity
.
Major
X-
ALD
phenotypes
are
the
progressive
childhood
cerebral
form
(
CCALD
)
with
early
confluent
cerebral
demyelination
and
the
adult-onset
adrenomyeloneuropathy
(
AMN
)
.
Adult
AMN
may
present
with
demyelinated
foci
of
the
CNS
(
adrenoleukomyeloneuropathy
,
ALMN
)
or
without
(
"
pure
"
AMN
)
.
Activated
methionine
is
essential
for
CNS
myelination
,
and
methionine
metabolism
is
important
for
glutathione
synthesis
,
which
may
influence
neurodegeneration
.
Cystathionine
beta
-synthase
(
CBS
)
is
a
key
enzyme
of
methionine
metabolism
.
The
CBS
variant
c
.
844
_
845
ins
68
(
p
.
-
)
may
influence
the
availability
of
activated
methionine
as
well
as
of
glutathione
.
In
this
study
,
we
analyzed
this
variant
in
genomic
DNA
samples
of
86
X-
ALD
patients
.
We
observed
the
allele
carrying
the
insertion
in
12
of
49
patients
without
CNS
demyelination
(
"
pure
"
AMN
)
,
but
in
none
of
the
37
patients
with
CNS
demyelination
(
CCALD
or
ALMN
;
chi
(
2
)
=
10
.
531
;
p
=
0
.
001
)
.
We
conclude
that
the
insertion
allele
of
CBS
c
.
844
_
845
ins
68
protected
X-
ALD
patients
against
CNS
demyelination
in
our
study
sample
.
These
data
suggest
that
the
individual
conditions
in
methionine
metabolism
may
be
a
disease
modifier
of
X-
ALD
.
Since
methionine
metabolism
can
easily
be
influenced
by
vitamin
and
amino
acid
substitution
,
this
observation
could
be
a
basis
of
novel
treatment
strategies
in
this
yet
untreatable
disease
.
(
c
)
2006
Wiley
-
Liss
,
Inc
.
Diseases
Validation
Diseases presenting
"cns demyelination"
symptom
adrenomyeloneuropathy
krabbe disease
This symptom has already been validated