Aberrant reduction of MiR-141 increased CD47/CUL3 in Hirschsprung's disease.

[hirschsprung disease]

MiR-141 has been confirmed to be associated with various human diseases . However , whether miR-141 is involved in the pathogenesis of Hirschsprung 's disease (HSCR ) remains unknown . Here , we design the experiment to reveal the relationship between miR-141 and HSCR .Quantitative real-time PCR and Western blot were used to detect the expression levels of miR-141 and its potential genes in 70 tissues of HSCR compared with 60 controls . Bisulfite sequencing PCR (BSP ) assay was applied to explain the possible mechanism of the aberrant expression level of miR-141 . We employed a dual-luciferase reporter assay to validate the regulation relation between miR-141 and CD 47 /CUL 3 . Cell migration , proliferation , apoptosis , and cell cycle progression were examined by transwell assay , MTT assay , and flow cytometry , respectively .M iR-141 was down-regulated whereas CD 47 and CUL 3 expression was increased in colon tissues from patients with HSCR compared with control group , The increased level of CD 47 and CUL 3 induced by miR-141 reduced proliferation and migration of 293 T and SH-SY 5 Y cells . Furthermore , this suppression was reversed by reducing of CD 47 and CUL 3 . Hypermethylation of a CpG Island in the promoter region of miR-141 gene was confirmed in HSCR tissues .Aberrant reduction of miR-141 may play an important role in the pathogenesis of HSCR with the inhibiting affection on cell migration and proliferation abilities . The present study demonstrates for the first time the role of miR-141 and its target genes in the occurrence of HSCR , and provides us a new direction for the study of the pathogenesis of Hirschsprung 's disease .

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Diseases presenting "reduction of mir-141" symptom

hirschsprung disease

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