Rare Diseases Symptoms Automatic Extraction
Home
A random Abstract
Our Project
Our Team
Purification of human wild-type or variant cystatin C from conditioned media of transfected cells.
[hereditary cerebral hemorrhage with amyloidosis]
The
characterization
of
proteins
in
their
native
state
is
essential
for
the
understanding
of
patho-genic
isoforms
.
A
variant
of
the
cysteine
protease
inhibitor
cystatin
C
is
the
major
constituent
of
the
amyloid
deposited
in
the
cerebral
vasculature
of
patients
with
the
Icelandic
form
of
hereditary
cerebral
hemorrhage
with
amyloidosis
(
HCHWA
-
I
)
.
In
order
to
study
the
nature
of
the
bio-physical
changes
owing
to
the
Leu
68
G
ln
substitution
in
cystatin
C
,
we
have
developed
a
purification
procedure
of
human
cystatin
C
in
its
native
state
.
The
protein
is
isolated
from
media
of
stably
transfected
tissue
culture
cells
using
physiological
conditions
that
preclude
protein
denaturation
.
The
importance
of
mild
purification
conditions
is
underscored
by
the
finding
that
denaturation
of
the
wild-
type
and
variant
proteins
facilitates
a
similar
folding
of
both
molecules
,
diminishing
their
differences
in
structure
and
biophysical
properties
.
Following
native
purification
conditions
,
variant
cystatin
C
has
a
distinct
structure
compared
to
the
wild-
type
protein
.
Diseases
Validation
Diseases presenting
"conditioned media of transfected cells.the characterization"
symptom
hereditary cerebral hemorrhage with amyloidosis
You can validate or delete this automatically detected symptom
Validate the Symptom
Delete the Symptom