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Early oxidative damage underlying neurodegeneration in X-adrenoleukodystrophy.
[adrenomyeloneuropathy]
X-
linked
adrenoleukodystrophy
(
X-
ALD
)
is
a
fatal
neurodegenerative
disorder
,
characterized
by
progressive
cerebral
demyelination
cerebral
childhood
adrenoleukodystrophy
(
CCALD
)
or
spinal
cord
neurodegeneration
(
adrenomyeloneuropathy
,
AMN
)
,
adrenal
insufficiency
and
accumulation
of
very
long
-chain
fatty
acids
(
VLCFA
)
in
tissues
.
The
disease
is
caused
by
mutations
in
the
ABCD
1
gene
,
which
encodes
a
peroxisomal
transporter
that
plays
a
role
in
the
import
of
VLCFA
or
VLCFA-CoA
into
peroxisomes
.
The
Abcd
1
knockout
mice
develop
a
spinal
cord
disease
that
mimics
AMN
in
adult
patients
,
with
late
onset
at
20
months
of
age
.
The
mechanisms
underlying
cerebral
demyelination
or
axonal
degeneration
in
spinal
cord
are
unknown
.
Here
,
we
present
evidence
by
gas
chromatography
/
mass
spectrometry
that
malonaldehyde-lysine
,
a
consequence
of
lipoxidative
damage
to
proteins
,
accumulates
in
the
spinal
cord
of
Abcd
1
knockout
mice
as
early
as
3
.
5
months
of
age
.
At
12
months
,
Abcd
1
-
mice
accumulate
additional
proteins
modified
by
oxidative
damage
arising
from
metal-catalyzed
oxidation
and
glycoxidation
/
lipoxidation
.
While
we
show
that
VLCFA
excess
activates
enzymatic
antioxidant
defenses
at
the
protein
expression
levels
,
both
in
neural
tissue
,
in
ex
vivo
organotypic
spinal
cord
slices
from
Abcd
1
-
mice
,
and
in
human
ALD
fibroblasts
,
we
also
demonstrate
that
the
loss
of
Abcd
1
gene
function
hampers
oxidative
stress
homeostasis
.
We
find
that
the
alpha-tocopherol
analog
Trolox
is
able
to
reverse
oxidative
lesions
in
vitro
,
thus
providing
therapeutic
hope
.
These
results
pave
the
way
for
the
identification
of
therapeutic
targets
that
could
reverse
the
deregulated
response
to
oxidative
stress
in
X-
ALD
.
Diseases
Validation
Diseases presenting
"adrenal insufficiency"
symptom
adrenal incidentaloma
adrenomyeloneuropathy
aromatase deficiency
congenital adrenal hyperplasia
cushing syndrome
familial hypocalciuric hypercalcemia
neonatal adrenoleukodystrophy
scrub typhus
triple a syndrome
von hippel-lindau disease
x-linked adrenoleukodystrophy
This symptom has already been validated