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Peroxisomal multifunctional protein-2 deficiency causes neuroinflammation and degeneration of Purkinje cells independent of very long chain fatty acid accumulation.
[adrenomyeloneuropathy]
Although
peroxisome
biogenesis
and
β-oxidation
disorders
are
well
known
for
their
neurodevelopmental
defects
,
patients
with
these
disorders
are
increasingly
diagnosed
with
neurodegenerative
pathologies
.
In
order
to
investigate
the
cellular
mechanisms
of
neurodegeneration
in
these
patients
,
we
developed
a
mouse
model
lacking
multifunctional
protein
2
(
MFP
2
,
also
called
D-
bifunctional
protein
)
,
a
central
enzyme
of
peroxisomal
β-oxidation
,
in
all
neural
cells
(
Nestin-
Mfp
2
(
-
/
-
)
)
or
in
oligodendrocytes
(
Cnp-
Mfp
2
(
-
/
-
)
)
and
compared
these
models
with
an
already
established
general
Mfp
2
knockout
.
Nestin-
Mfp
2
but
not
Cnp-
Mfp
2
knockout
mice
develop
motor
disabilities
and
ataxia
,
similar
to
the
general
mutant
.
Deterioration
of
motor
performance
correlates
with
the
demise
of
Purkinje
cell
axons
in
the
cerebellum
,
which
precedes
loss
of
Purkinje
cells
and
cerebellar
atrophy
.
This
closely
mimics
spinocerebellar
ataxias
of
patients
affected
with
mild
peroxisome
β-oxidation
disorders
.
However
,
general
knockouts
have
a
much
shorter
life
span
than
Nestin-
Mfp
2
knockouts
which
is
paralleled
by
a
disparity
in
activation
of
the
innate
immune
system
.
Whereas
in
general
mutants
a
strong
and
chronic
proinflammatory
reaction
proceeds
throughout
the
brain
,
elimination
of
MFP
2
from
neural
cells
results
in
minor
neuroinflammation
.
Neither
the
extent
of
the
inflammatory
reaction
nor
the
cerebellar
degeneration
could
be
correlated
with
levels
of
very
long
chain
fatty
acids
,
substrates
of
peroxisomal
β-oxidation
.
In
conclusion
,
MFP
2
has
multiple
tasks
in
the
adult
brain
,
including
the
maintenance
of
Purkinje
cells
and
the
prevention
of
neuroinflammation
but
this
is
not
mediated
by
its
activity
in
oligodendrocytes
nor
by
its
role
in
very
long
chain
fatty
acid
degradation
.
Diseases
Validation
Diseases presenting
"innate immune system"
symptom
adrenomyeloneuropathy
allergic bronchopulmonary aspergillosis
familial mediterranean fever
inclusion body myositis
legionellosis
primary effusion lymphoma
typhoid
x-linked adrenoleukodystrophy
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