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Dysregulated mature IL-1β production in familial Mediterranean fever.
[familial mediterranean fever]
The
aim
of
this
study
was
to
analyse
the
role
of
circulating
cleaved
IL
-
1
β
in
patients
with
FMF
.
We
enrolled
20
patients
with
FMF
(
5
males
and
15
females
)
,
22
patients
with
RA
(
4
males
and
18
females
)
and
22
healthy
controls
(
6
males
and
16
females
)
.
Serum
levels
of
serum
amyloid
A
(
SAA
)
were
measured
by
ELISA
.
We
also
determined
whether
IL
-
1
β
was
present
as
the
cleaved
form
(
p
17
)
in
the
sera
of
FMF
patients
by
immunoblotting
using
anti-cleaved
IL
-
1
β
antibody
.
Although
SAA
concentrations
were
elevated
in
the
sera
,
there
was
no
significant
difference
in
these
concentrations
between
FMF
patients
and
RA
patients
.
Immunoblot
analysis
demonstrated
that
the
cleaved
form
of
IL
-
1
β
(
p
17
)
was
present
in
sera
from
FMF
patients
during
febrile
attack
periods
,
but
not
in
healthy
controls
.
Bands
representing
the
cleaved
form
of
IL
-
1
β
were
not
detected
in
serum
from
FMF
patients
at
non-febrile
attack
periods
or
remission
periods
under
colchicine
treatment
.
The
amounts
of
cleaved
IL
-
1
β
(
p
17
)
were
significantly
higher
in
patients
with
FMF
compared
with
those
in
patients
with
RA
in
the
inflammatory
phase
.
The
cleaved
form
of
IL
-
1
β
is
a
valuable
biomarker
for
monitoring
disease
activity
and
response
to
colchicine
treatment
in
patients
with
FMF
.
It
might
be
useful
to
discriminate
FMF
from
other
non-
IL
-
1
β-mediated
inflammatory
disorders
.
Diseases
Validation
Diseases presenting
"β-mediated inflammatory disorders"
symptom
familial mediterranean fever
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