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Pain related channels are differentially expressed in neuronal and non-neuronal cells of glabrous skin of fabry knockout male mice.
[fabry disease]
Fabry
disease
(
FD
)
is
one
of
the
X-
linked
lysosomal
storage
disorders
caused
by
deficient
functioning
of
the
alpha-galactosidase
A
(
α-
GalA
)
enzyme
.
The
α-
GalA
deficiency
leads
to
multi-systemic
clinical
manifestations
caused
by
the
preferential
accumulation
of
globotriaosylceramide
in
the
endothelium
and
vascular
smooth
muscles
.
A
hallmark
symptom
of
FD
patients
is
peripheral
pain
that
appears
in
the
early
stage
of
the
disease
.
Pain
in
FD
patients
is
a
peripheral
small
-fiber
idiopathic
neuropathy
,
with
intra-epidermal
fiber
density
and
integrity
being
used
for
diagnosing
FD
in
humans
.
However
,
the
molecular
correlates
underlying
pain
sensation
in
FD
remain
elusive
.
Here
,
we
have
employed
the
α-
GalA
gene
KO
mouse
as
a
model
of
FD
in
rodents
to
investigate
molecular
changes
in
their
peripheral
nervous
system
that
may
account
for
their
algesic
symptoms
.
The
α-
GalA
null
mice
display
neuropathic
pain
as
evidenced
by
thermal
hyperalgesia
and
mechanical
allodynia
,
with
histological
analyses
showing
alterations
in
cutaneous
innervation
.
Additionally
,
KO
mice
showed
a
decreased
and
scattered
pattern
of
neuronal
terminations
consistent
with
the
reduction
in
neuronal
terminations
in
skin
biopsies
of
patients
with
small
fiber
neuropathies
.
At
the
molecular
level
KO
animals
showed
an
increase
in
the
expression
of
TRPV
1
and
Nav
1
.
8
,
and
a
decrease
in
the
expression
of
TRPM
8
.
Notably
,
these
alterations
are
observed
in
young
animals
.
Taken
together
,
our
findings
imply
that
the
α-
GalA
KO
mouse
is
a
good
model
in
which
to
study
the
peripheral
small
fiber
neuropathy
exhibited
by
FD
patients
,
and
provides
molecular
evidence
for
a
hyperexcitability
of
small
nociceptors
in
FD
.
Diseases
Validation
Diseases presenting
"peripheral small fiber neuropathy"
symptom
fabry disease
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