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A random Abstract
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Exploration of potential roles of a new LOXL2 splicing variant using network knowledge in esophageal squamous cell carcinoma.
[esophageal squamous cell carcinoma]
LOXL
2
(
lysyl
oxidase-like
2
)
,
an
enzyme
that
catalyzes
oxidative
deamination
of
lysine
residue
,
is
upregulated
in
esophageal
squamous
cell
carcinoma
(
ESCC
)
.
A
LOXL
2
splice
variant
LOXL
2
-
e
13
and
its
wild
type
were
overexpressed
in
ESCC
cells
followed
by
microarray
analyses
.
In
this
study
,
we
explored
the
potential
role
and
molecular
mechanism
of
LOXL
2
-
e
13
based
on
known
protein-protein
interactions
(
PPIs
)
,
following
microarray
analysis
of
KYSE
150
ESCC
cells
overexpressing
a
LOXL
2
splice
variant
,
denoted
by
LOXL
2
-
e
13
,
or
its
wild-
type
counterpart
.
The
differentially
expressed
genes
(
DEGs
)
of
LOXL
2
-
WT
and
LOXL
2
-
e
13
were
applied
to
generate
individual
PPI
subnetworks
in
which
hundreds
of
DEGs
interacted
with
thousands
of
other
proteins
.
These
two
DEG
groups
were
annotated
by
Functional
Annotation
Chart
analysis
in
the
DAVID
bioinformatics
database
and
compared
.
These
results
found
many
specific
annotations
indicating
the
potential
specific
role
or
mechanism
for
LOXL
2
-
e
13
.
The
DEGs
of
LOXL
2
-
e
13
,
comparing
to
its
wild
type
,
were
prioritized
by
the
Random
Walk
with
Restart
algorithm
.
Several
tumor
-related
genes
such
as
ERO
1
L
,
ITGA
3
,
and
MAPK
8
were
found
closest
to
LOXL
2
-
e
13
.
These
results
provide
helpful
information
for
subsequent
experimental
identification
of
the
specific
biological
roles
and
molecular
mechanisms
of
LOXL
2
-
e
13
.
Our
study
also
provides
a
work
flow
to
identify
potential
roles
of
splice
variants
with
large
scale
data
.
Diseases
Validation
Diseases presenting
"squamous cell carcinoma"
symptom
carcinoma of the gallbladder
child syndrome
dystrophic epidermolysis bullosa
epidermolysis bullosa simplex
esophageal adenocarcinoma
esophageal carcinoma
esophageal squamous cell carcinoma
junctional epidermolysis bullosa
kallmann syndrome
kindler syndrome
liposarcoma
monosomy 21
oculocutaneous albinism
oral submucous fibrosis
papillon-lefèvre syndrome
This symptom has already been validated