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Expression and clinical role of NF45 as a novel cell cycle protein in esophageal squamous cell carcinoma (ESCC).
[esophageal squamous cell carcinoma]
NF
45
(
also
known
as
ILF
2
)
,
as
one
subunit
of
NF-AT
(
nuclear
factor
of
activated
T
cells
)
,
repairs
DNA
breaks
,
inhibits
viral
replication
,
and
also
functions
as
a
negative
regulator
in
the
microRNA
processing
pathway
in
combination
with
NF
90
.
Recently
,
it
was
found
that
implicated
in
the
mitotic
control
of
HeLa
cells
and
deletion
of
endogenous
NF
45
decreases
growth
of
HeLa
cells
.
While
the
role
of
NF
45
in
cancer
biology
remains
under
debate
.
In
this
study
,
we
analyzed
the
expression
and
clinical
significance
of
NF
45
in
esophageal
squamous
cell
carcinoma
ESCC
.
The
expression
of
NF
45
was
evaluated
by
Western
blot
in
8
paired
fresh
ESCC
tissues
and
immunohistochemistry
on
105
paraffin-embedded
slices
.
NF
45
was
highly
expressed
in
ESCC
and
significantly
associated
with
ESCC
cells
tumor
stage
and
Ki-
67
.
Besides
,
high
NF
45
expression
was
an
independent
prognostic
factor
for
ESCC
patients
'
poor
survival
.
To
determine
whether
NF
45
could
regulate
the
proliferation
of
ESCC
cells
,
we
increased
endogenous
NF
45
and
analyzed
the
proliferation
of
TE
1
ESCC
cells
using
Western
blot
,
CCK
8
,
flow
cytometry
assays
and
colony
formation
analyses
,
which
together
indicated
that
overexpression
of
NF
45
favors
cell
cycle
progress
of
TE
1
ESCC
cells
.
While
knockdown
of
NF
45
resulted
in
cell
cycle
arrest
at
G
0
/
G
1
-
phase
and
thus
abolished
the
cell
growth
.
These
findings
suggested
that
NF
45
might
play
an
important
role
in
promoting
the
tumorigenesis
of
ESCC
,
and
thus
be
a
promising
therapeutic
target
to
prevent
ESCC
progression
.
Diseases
Validation
Diseases presenting
"promising therapeutic target"
symptom
esophageal squamous cell carcinoma
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