Rare Diseases Symptoms Automatic Extraction
Home
A random Abstract
Our Project
Our Team
[Genome wide screening and characterization of long non-coding RNAs in esophageal cancer].
[esophageal squamous cell carcinoma]
To
screen
for
esophageal
squamous
cell
carcinoma
(
ESCC
)
-
associated
long
non-coding
RNAs
(
lncRNA
)
and
identify
oncogenic
lncRNA
contributing
to
ESCC
pathogenesis
.
A
lncRNA
array
containing
7419
lncRNA
was
used
to
detect
the
transcriptional
profiles
of
lncRNA
of
four
pairs
of
ESCC
and
matched
normal
esophageal
tissue
.
Bioinformatic
analysis
was
employed
to
identify
differentially
expressed
ESCC
associated
lncRNA
(
ESCCAL
)
.
Quantitative
real-time
PCR
was
used
to
verify
selected
dysregulated
lncRNA
on
independent
ESCC
samples
.
Genome-
wide
transcriptome
profiling
(
coding
and
or
noncoding
RNA
transcripts
)
was
able
to
distinguish
ESCC
from
normal
tissue
.
Among
these
,
bioinformatic
analysis
has
identified
154
differentially
expressed
ESCC
associated
lncRNA
(
ESCCALs
)
,
which
included
111
downregulated
and
43
upregulated
lncRNA
in
ESCC
relative
to
the
normal
tissue
(
P
<
0
.
01
)
.
The
highest
upregulated
lncRNA
(
ESCCAL
_
1
)
and
known
onco-lnc
RNA
HOTAIR
was
further
verified
in
26
paired
ESCC
samples
.
ESCCAL
_
1
and
HOTAIR
were
found
to
be
highly
expressed
in
17
ESCC
and
18
ESCC
compared
with
normal
esophageal
tissues
.
This
investigation
has
revealed
large
scale
aberrant
expression
of
lncRNA
in
ESCC
.
About
70
%
of
novel
lncRNA-ESCCAL
_
1
,
together
with
a
known
lncRNA-HOTAIR
,
are
highly
expressed
in
ESSC
,
suggesting
that
ESCCAL
_
1
and
HOTAIR
may
participate
in
the
pathological
process
of
ESCC
.
Furthermore
,
lncRNA
could
be
potential
diagnostic
and
prognostic
biomarkers
for
ESCC
.