Screening aberrant methylation profile in esophageal squamous cell carcinoma for Kazakhs in Xinjiang area of China.

[esophageal squamous cell carcinoma]

To screen the aberrant methylation genes in esophageal squamous cell carcinoma (ESCC ) for Kazakh nationality in Xinjiang , and the aberrant DNA methylation genes pattern provides a clue for deeply study on ESCC mechanism . Illumina Human Methylation 450 Â K chip was used to screen the genome-wide methylation on six cancer tissues and six adjacent normal tissues of ESCC in Kazakh people . Meanwhile , mRNA library was constructed by scanning the RNA expression on two cancer tissues and two adjacent normal tissues by Hiseq 2000 . After association study between the methylation profile and expression profile , aberrant DNA methylated genes were screened out and were uploaded to the GoMiner and the KEGG , completing the bioinformatic analysis . There were 227 hypermethylation genes and 6 hypomethylated genes in cancer tissue , mRNA expression varied from 0 .0312 to 8 ,192 in cancer tissues compared with 0 .0312 -1 ,024 in adjacent normal tissues . The correlation study indicated that there were 10 loci in 10 down-regulated genes of hypermethylated in negative correlation group . Additionally , there were 11 loci in 10 up-regulated genes in negative group . Using GoMiner to do GO analysis on aberrant DNA methylation genes , RAPGEFL 1 , TP 53 AIP 1 , KIAA 1522 , DUOXA 2 were identified not involved in any biological processes . ALDH 1 L 1 participated in folinic acid catabolism and CAPN 1 positively regulated the cell proliferation . And ALDH 1 L 1 involved in one carbon metabolism and CAPN 1 participate in the apoptosis process by applying pathway analysis . The aberrant DNA methylation profiles were established and provided a clue for deeply study on ESCC of Kazakh nationality .

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esophageal squamous cell carcinoma

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