High expression of long non-coding RNA SPRY4-IT1 predicts poor prognosis of clear cell renal cell carcinoma.

[esophageal squamous cell carcinoma]

Long non-coding RNAs (lncRNAs ) play a key role in cellular processes , such as cell growth , apoptosis , and carcinogenesis . lncRNAs SPRY 4 -IT 1 has recently been identified to be involved in tumorigenesis of several cancers such as non-small cell lung cancer and esophageal squamous cell carcinoma . However , the role of SPRY 4 -IT 1 in clear cell renal cell carcinoma (ccRCC ) remains unclear .The expression of SPRY 4 -IT 1 was examined in ccRCC patients and renal cancer cell lines by using quantitative real-time PCR (qRT-PCR ). The relationship between SPRY 4 -IT 1 level and clinicopathological parameters of ccRCC was analyzed with the Kaplan-Meier method and Cox proportional hazards model . Small interfering RNA (siRNA ) was used to suppress SPRY 4 -IT 1 expression in renal cancer cell line 786 -O . In vitro assays were performed to further explore its role in renal cancer progressio .The relative level of SPRY 4 -IT 1 was significantly higher in ccRCC tissues compared to the adjacent normal renal tissues . And higher expression of SPRY 4 -IT 1 was found in renal cancer cell lines compared with the normal human proximal tubule epithelial cell line HK -2 . The ccRCC patients with higher SPRY 4 -IT 1 expression had an advanced clinical stage and poorer prognosis than those with lower SPRY 4 -IT 1 expression . Multivariate analyses by Cox 's proportional hazard model revealed that expression of SPRY 4 -IT 1 was an independent prognostic factor in ccRCC . In vitro assays , our results indicated that knockdown of SPRY 4 -IT 1 reduced renal cancer cell proliferation , migration , and invasion .Our data suggested that lncRNA SPRY 4 -IT 1 might be considered as a potential prognostic indicator and a potential target for therapeutic intervention in RC .

Diseases
Validation

Diseases presenting "cancer" symptom

achondroplasia

acute rheumatic fever

adrenal incidentaloma

alpha-thalassemia

benign recurrent intrahepatic cholestasis

cadasil

canavan disease

carcinoma of the gallbladder

cholangiocarcinoma

coats disease

congenital adrenal hyperplasia

congenital diaphragmatic hernia

cowden syndrome

cushing syndrome

cutaneous mastocytosis

dedifferentiated liposarcoma

dystrophic epidermolysis bullosa

epidermolysis bullosa simplex

erdheim-chester disease

erythropoietic protoporphyria

esophageal adenocarcinoma

esophageal carcinoma

esophageal squamous cell carcinoma

familial hypocalciuric hypercalcemia

familial mediterranean fever

gm1 gangliosidosis

heparin-induced thrombocytopenia

hereditary cerebral hemorrhage with amyloidosis

hirschsprung disease

hodgkin lymphoma, classical

inclusion body myositis

junctional epidermolysis bullosa

kabuki syndrome

kallmann syndrome

kindler syndrome

lamellar ichthyosis

liposarcoma

locked-in syndrome

lymphangioleiomyomatosis

monosomy 21

neuralgic amyotrophy

oculocutaneous albinism

oligodontia

oral submucous fibrosis

papillon-lefèvre syndrome

pendred syndrome

pleomorphic liposarcoma

primary effusion lymphoma

proteus syndrome

pyomyositis

pyruvate dehydrogenase deficiency

severe combined immunodeficiency

sneddon syndrome

systemic capillary leak syndrome

triple a syndrome

von hippel-lindau disease

waldenström macroglobulinemia

well-differentiated liposarcoma

werner syndrome

wiskott-aldrich syndrome

wolf-hirschhorn syndrome

x-linked adrenoleukodystrophy

This symptom has already been validated