Epigenetic regulation of CD271, a potential cancer stem cell marker associated with chemoresistance and metastatic capacity.

[esophageal squamous cell carcinoma]

Cancer stem cells (CSCs ) are considered to be the cause of tumor initiation , metastasis and recurrence . Additionally , CSCs are responsible for the failure of chemotherapy and radiotherapy . The isolation and identification of CSCs is crucial for facilitating the monitoring , therapy or prevention of cancer . We aimed to identify esophageal squamous cell carcinoma (ESCC ) stem-like cells , the epigenetic mechanism and identify novel biomarkers for targeting ESCC CSCs . Sixty -three paired ESCC tissues and adjacent non-cancerous tissues were included in this study . CD 27 1 , which was identified as the CSC marker for melanoma , was assessed using quantitative PCR (qPCR ). Using flow cytometry , we isolated CD 27 1 + cells comprising 7 .5 % of cancer cells from the KYSE 70 cell line . Sphere formation and anchorage -independent growth were analyzed in CD 27 1 + and CD 27 1 - cancer cells , respectively . qPCR was used to detect stem-related genes and CCK -8 was performed to analyze the sensitivity to chemotherapy in the two groups . Bisulﬁte genomic sequencing was used to analyze the methylation status . CD 27 1 expression was significantly higher in ESCC tissues than in adjacent non-cancerous tissues . Compared with CD 27 1 - cancer cells , CD 27 1 + cancer cells showed a higher ability of sphere and colony formation , a high level expression of stem-related gene , and resistance to chemotherapy . The expression of CD 27 1 was induced by a demethylation agent . In conclusion , CD 27 1 + ESCC cells possess stem-like properties . CD 27 1 can potentially act as a prognostic marker for ESCC , whose expression is regulated epigenetically .

Diseases
Validation

Diseases presenting "cancer" symptom

achondroplasia

acute rheumatic fever

adrenal incidentaloma

alpha-thalassemia

benign recurrent intrahepatic cholestasis

cadasil

canavan disease

carcinoma of the gallbladder

cholangiocarcinoma

coats disease

congenital adrenal hyperplasia

congenital diaphragmatic hernia

cowden syndrome

cushing syndrome

cutaneous mastocytosis

dedifferentiated liposarcoma

dystrophic epidermolysis bullosa

epidermolysis bullosa simplex

erdheim-chester disease

erythropoietic protoporphyria

esophageal adenocarcinoma

esophageal carcinoma

esophageal squamous cell carcinoma

familial hypocalciuric hypercalcemia

familial mediterranean fever

gm1 gangliosidosis

heparin-induced thrombocytopenia

hereditary cerebral hemorrhage with amyloidosis

hirschsprung disease

hodgkin lymphoma, classical

inclusion body myositis

junctional epidermolysis bullosa

kabuki syndrome

kallmann syndrome

kindler syndrome

lamellar ichthyosis

liposarcoma

locked-in syndrome

lymphangioleiomyomatosis

monosomy 21

neuralgic amyotrophy

oculocutaneous albinism

oligodontia

oral submucous fibrosis

papillon-lefèvre syndrome

pendred syndrome

pleomorphic liposarcoma

primary effusion lymphoma

proteus syndrome

pyomyositis

pyruvate dehydrogenase deficiency

severe combined immunodeficiency

sneddon syndrome

systemic capillary leak syndrome

triple a syndrome

von hippel-lindau disease

waldenström macroglobulinemia

well-differentiated liposarcoma

werner syndrome

wiskott-aldrich syndrome

wolf-hirschhorn syndrome

x-linked adrenoleukodystrophy

This symptom has already been validated