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Silencing of insulin-like growth factor-1 receptor enhances the radiation sensitivity of human esophageal squamous cell carcinoma in vitro and in vivo.
[esophageal squamous cell carcinoma]
Esophageal
squamous
cell
carcinoma
(
ESCC
)
is
a
prevalent
fatal
cancer
worldwide
,
and
the
number
of
deaths
due
to
this
disease
is
increasing
.
Due
to
ESCC
resistance
to
chemotherapy
and
radiation
treatment
,
new
therapies
are
urgently
needed
for
the
improvement
of
ESCC
patient
clinical
outcomes
.
Eca-
109
and
TE-
1
cells
were
transfected
with
100
nM
IGF-
1
r
siRNA
,
and
a
combination
of
IGF-
1
r
siRNA
and
radiation
therapy
was
tested
in
vitro
and
in
vivo
.
The
effects
of
IGF-
1
r
siRNA
were
determined
through
Western
blotting
and
flow
cytometry
experiments
.
After
radiotherapy
,
the
number
of
IGF-
1
r
siRNA-transfected
Eca-
109
cells
decreased
by
approximately
67
.
3
%
,
and
a
78
.
9
%
reduction
was
observed
in
the
transfected
TE-
1
cells
.
In
addition
,
the
Eca-
109
and
TE-
1
cells
that
were
irradiated
following
IGF-
1
r
knockdown
contained
16
.
2
%
and
20
.
3
%
apoptotic
cells
,
respectively
.
The
results
of
the
current
study
suggest
that
IGF-
1
r
knockdown
may
enhance
the
radiation
sensitivity
of
ESCC
and
increase
the
therapeutic
effects
of
radiation
both
in
vitro
and
in
vivo
.
These
results
provide
strong
evidence
that
the
targeted
application
of
siRNA
will
enable
the
development
of
new
therapeutic
strategies
for
the
clinical
treatment
of
ESCC
patients
.