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A novel human interleukin-24 peptide created by computer-guided design contributes to suppression of proliferation in esophageal squamous cell carcinoma Eca-109 cells.
[esophageal squamous cell carcinoma]
Based
on
the
three
-dimensional
modeling
structure
of
human
interleukin-
24
(
hIL-
24
)
and
its
most
likely
active
position
predicted
by
solvent
accessibility
and
apparent
electrostatic
properties
,
a
novel
hIL-
24
peptide
M
1
was
created
by
computer-guided
molecular
design
.
The
cytotoxicity
and
cell
selectivity
of
M
1
were
examined
in
three
human
carcinoma
cell
lines
and
one
normal
human
embryo
lung
fibroblast
cell
line
(
HEL
)
.
MTT
assay
showed
that
M
1
induced
growth
arrest
in
two
IL
-
20
receptor
complex
-
positive
cancer
cell
lines
(
the
esophageal
squamous
cell
carcinoma
cell
line
Eca-
109
and
the
melanoma
cell
line
A
375
)
,
and
antibodies
against
IL
-
24
or
IL
-
20
receptor
complexes
significantly
neutralized
the
inhibitory
activity
.
Moreover
,
M
1
had
almost
no
cytotoxicity
on
the
lung
cancer
A
549
cell
line
,
which
lacks
a
full
complement
of
the
IL
-
20
receptor
complexes
,
or
on
HEL
cells
that
express
the
IL
-
20
receptor
complexes
.
These
findings
demonstrate
that
M
1
could
act
as
an
excellent
candidate
for
the
induction
of
growth
arrest
on
receptor
complex
-
positive
cancer
cells
.
In
summary
,
the
M
1
peptide
may
represent
a
novel
anticancer
agent
for
esophageal
squamous
cell
carcinoma
therapy
due
to
its
cancer
cell
selectivity
and
its
relatively
low
cytotoxicity
to
normal
cells
.