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[Amphotericin B suppresses migration and invasion of esophageal carcinoma Eca109 cells in hypoxic microenvironment by down-regulating hypoxia-inducible factor-1α activity].
[esophageal carcinoma]
To
investigate
the
effect
of
amphotericinB
(
AmB
)
on
migration
and
invasion
of
esophageal
carcinoma
Eca
109
cells
exposed
to
hypoxia
and
explore
the
molecular
mechanisms
.
Routinely
cultured
esophageal
carcinoma
Eca
109
cells
were
treated
with
0
,
1
.
25
,
2
.
5
,
or
5
µg
/
ml
AmB
in
hypoxic
condition
(
3
%
O
2
,
5
%
CO
2
,
and
92
%
N
2
)
for
24
h
.
The
cell
migration
and
invasion
were
assessed
by
cell
scratch
test
and
Transwell
chamber
assay
,
respectively
.
Real-time
quantitative
PCR
and
Western
blotting
were
used
to
detect
the
mRNA
and
protein
expressions
of
hypoxia-inducible
factor
-
1
α
(
HIF-
1
α
)
,
matrix
metalloproteinase-
2
(
MMP-
2
)
,
and
E
-
cadherin
in
the
cells
,
respectively
.
Compared
with
the
control
cells
,
the
cells
treated
with
different
doses
of
AmB
showed
attenuated
ability
of
migration
and
invasion
(
P
<
0
.
05
)
.
AmB
treatment
resulted
in
significantly
lowered
mRNA
and
protein
expressions
of
MMP-
2
(
P
<
0
.
05
)
and
increased
expressions
of
E
-
cadherin
(
P
<
0
.
05
)
;
the
protein
expression
of
HIF-
1
α
decreased
significantly
in
cells
after
AmB
treatment
(
P
<
0
.
05
)
but
its
mRNA
levels
showed
no
significant
changes
(
P
>
0
.
05
)
.
AmB
can
suppress
the
migration
and
invasion
of
esophageal
carcinoma
Eca
109
cells
in
hypoxic
microenvironment
possibly
by
regulating
the
expressions
of
HIF-
1
α
,
MMP-
2
and
E
-
cadherin
.
Diseases
Validation
Diseases presenting
"protein expressions of mmp-2"
symptom
esophageal carcinoma
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