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Enhancement of silencing DNA polymerase β on the radiotherapeutic sensitivity of human esophageal carcinoma cell lines.
[esophageal carcinoma]
Human
DNA
polymerase
β
(
DNA
polymerase
β
(
polβ
)
)
is
a
small
monomeric
protein
which
is
essential
for
short
-patch
base
excision
repair
(
BER
)
.
It
plays
an
important
role
in
regulating
the
radiation
sensitivity
of
tumor
cells
in
the
course
of
tumor
radiation
therapy
.
In
this
study
,
qRT-PCR
and
Western
blot
assays
were
used
to
quantify
polβ
expression
levels
in
esophageal
carcinoma
(
EC
)
cells
that
were
transfected
with
pol
β
small
interfering
RNA
(
siRNA
)
.
Cell
counting
Kit-
8
(
CCK
-
8
)
,
flow
cytometry
,
and
Hoechst
/
PI
stain
assays
were
conducted
to
evaluate
the
effects
of
silencing
polβ
on
the
radiotherapeutic
sensitivity
of
EC
cells
.
We
found
that
the
expression
levels
of
polβ
in
EC
cells
were
significantly
decreased
after
transfection
with
polβ
siRNA
.
Then
,
we
found
that
polβ
silencing
increased
the
sensitivity
of
EC
cells
to
radiation
therapy
.
In
conclusion
,
our
study
paves
the
way
for
a
better
understanding
of
the
mechanism
of
the
polβ
gene
in
DNA
repair
,
and
we
propose
that
RNA
interference
technology
will
have
important
applications
in
gene
therapy
of
EC
and
other
cancers
in
the
future
.
