DADS suppresses human esophageal xenograft tumors through RAF/MEK/ERK and mitochondria-dependent pathways.

[esophageal carcinoma]

Diallyl disulfide (DADS ) is a natural organosulfur compound isolated from garlic . DADS has various biological properties , including anticancer , antiangiogenic , and antioxidant effects . However , the anticancer mechanisms of DADS in human esophageal carcinoma have not been elucidated , especially in vivo . In this study , MTT assay showed that DADS significantly reduced cell viability in human esophageal carcinoma ECA 109 cells , but was relatively less toxic in normal liver cells . The pro-apoptotic effect of DADS on ECA 109 cells was detected by Annexin V-FITC /propidium iodide (PI ) staining . Flow cytometry analysis showed that DADS promoted apoptosis in a dose-dependent manner and the apoptosis rate could be decreased by caspase-3 inhibitor Ac-DEVD-CHO . Xenograft study in nude mice showed that DADS treatment inhibited the growth of ECA 109 tumor in both 20 and 40 mg /kg DADS groups without obvious side effects . DADS inhibited ECA 109 tumor proliferation by down-regulating proliferation cell nuclear antigen (PCNA ). DADS induced apoptosis by activating a mitochondria-dependent pathway with the executor of caspase-3 , increasing p 53 level and Bax /Bcl-2 ratio , and downregulating the RAF /MEK /ERK pathway in ECA 109 xenograft tumosr . Based on studies in cell culture and animal models , the findings here indicate that DADS is an effective and safe anti-cancer agent for esophageal carcinoma .

Diseases
Validation

Diseases presenting "cancer" symptom

achondroplasia

acute rheumatic fever

adrenal incidentaloma

alpha-thalassemia

benign recurrent intrahepatic cholestasis

cadasil

canavan disease

carcinoma of the gallbladder

cholangiocarcinoma

coats disease

congenital adrenal hyperplasia

congenital diaphragmatic hernia

cowden syndrome

cushing syndrome

cutaneous mastocytosis

dedifferentiated liposarcoma

dystrophic epidermolysis bullosa

epidermolysis bullosa simplex

erdheim-chester disease

erythropoietic protoporphyria

esophageal adenocarcinoma

esophageal carcinoma

esophageal squamous cell carcinoma

familial hypocalciuric hypercalcemia

familial mediterranean fever

gm1 gangliosidosis

heparin-induced thrombocytopenia

hereditary cerebral hemorrhage with amyloidosis

hirschsprung disease

hodgkin lymphoma, classical

inclusion body myositis

junctional epidermolysis bullosa

kabuki syndrome

kallmann syndrome

kindler syndrome

lamellar ichthyosis

liposarcoma

locked-in syndrome

lymphangioleiomyomatosis

monosomy 21

neuralgic amyotrophy

oculocutaneous albinism

oligodontia

oral submucous fibrosis

papillon-lefèvre syndrome

pendred syndrome

pleomorphic liposarcoma

primary effusion lymphoma

proteus syndrome

pyomyositis

pyruvate dehydrogenase deficiency

severe combined immunodeficiency

sneddon syndrome

systemic capillary leak syndrome

triple a syndrome

von hippel-lindau disease

waldenström macroglobulinemia

well-differentiated liposarcoma

werner syndrome

wiskott-aldrich syndrome

wolf-hirschhorn syndrome

x-linked adrenoleukodystrophy

This symptom has already been validated