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Expression of BAG-1 is closely related to cell differentiation and TNM stage in esophageal cancer and its downregulation inhibits the proliferation and invasion of human esophageal carcinoma cells.
[esophageal carcinoma]
The
aim
of
the
present
study
was
to
explore
the
correlation
of
BAG-
1
with
clinical
characteristics
of
esophageal
cancer
and
its
effects
on
the
proliferation
,
invasion
and
apoptosis
of
the
esophageal
carcinoma
cell
line
Eca
109
.
Therefore
,
the
expression
of
BAG-
1
was
assessed
in
esophageal
carcinoma
tumor
tissues
and
adjacent
normal
esophageal
tissues
.
The
siRNA
vector
of
BAG-
1
was
constructed
and
transfected
into
the
Eca
109
cell
line
,
and
then
fluorescence
microscopy
was
used
to
evaluate
the
transfection
efficiency
.
MTT
and
Transwell
assays
were
used
to
study
cell
proliferation
and
invasive
activity
,
and
the
apoptosis
rate
was
assessed
by
flow
cytometry
.
Western
blotting
was
adopted
to
assess
the
silencing
efficiency
and
expression
of
related
gene
bcl-
2
.
The
results
revealed
that
BAG-
1
expression
was
low
in
the
adjacent
normal
esophageal
tissues
while
expression
was
high
in
the
esophageal
carcinoma
tissues
.
After
Eca
109
cells
were
transfected
with
BAG-
1
-
siRNA
,
the
proliferation
and
invasive
capabilities
of
the
cells
were
significantly
decreased
while
the
apoptosis
rate
was
greatly
enhanced
(
P
<
0
.
01
)
.
When
the
expression
of
BAG-
1
in
the
Eca
109
cells
was
downregulated
,
the
expression
of
bcl-
2
was
significantly
abated
(
P
<
0
.
05
)
.
In
conclusion
,
BAG-
1
is
closely
connected
with
the
pathogenesis
and
development
of
esophageal
carcinoma
,
which
may
act
through
affecting
bcl-
2
.
Diseases
Validation
Diseases presenting
"related gene bcl-2. the results"
symptom
esophageal carcinoma
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