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CecropinXJ, a silkworm antimicrobial peptide, induces cytoskeleton disruption in esophageal carcinoma cells.
[esophageal carcinoma]
Antimicrobial
peptides
exist
in
the
non-
specific
immune
system
of
organism
and
participate
in
the
innate
host
defense
of
each
species
.
CecropinXJ
,
a
cationic
antimicrobial
peptide
,
possesses
potent
anticancer
activity
and
acts
preferentially
on
cancer
cells
instead
of
normal
cells
,
but
the
mechanism
of
cancer
cell
death
induced
by
cecropinXJ
remains
largely
unknown
.
This
study
was
performed
to
investigate
the
cytoskeleton-disrupting
effects
of
cecropinXJ
on
human
esophageal
carcinoma
cell
line
Eca
109
using
scanning
electron
microscopy
observation
,
fluorescence
imaging
,
cell
migration
and
invasion
assays
,
western
blotting
,
and
quantitative
reverse
transcription
polymerase
chain
reaction
(
qRT-PCR
)
analysis
.
The
electronic
microscope
and
fluorescence
imaging
observation
suggested
that
cecropinXJ
could
result
in
morphological
changes
and
induce
damage
to
microtubules
and
actin
of
Eca
109
cells
in
a
dose-dependent
manner
.
The
cell
migration
and
invasion
assays
demonstrated
that
cecropinXJ
could
inhibit
migration
and
invasion
of
tumor
cells
.
Western
blot
and
qRT-PCR
analysis
showed
that
there
was
obvious
correlation
between
microtubule
depolymerization
and
actin
polymerization
induced
by
cecropinXJ
.
Moreover
,
cecropinXJ
might
also
cause
decreased
expression
of
α-actin
,
β-actin
,
γ-actin
,
α-tubulin
,
and
β-tubulin
genes
in
concentration-
and
time-dependent
manners
.
In
summary
,
this
study
indicates
that
cecropinXJ
triggers
cytotoxicity
in
Eca
109
cells
through
inducing
the
cytoskeleton
destruction
and
regulating
the
expression
of
cytoskeleton
proteins
.
This
cecropinXJ-mediated
cytoskeleton-destruction
effect
is
instrumental
in
our
understanding
of
the
detailed
action
of
antimicrobial
peptides
in
human
cancer
cells
and
cecropinXJ
might
be
a
potential
therapeutic
agent
for
the
treatment
of
cancer
in
the
future
.
Diseases
Validation
Diseases presenting
"potential therapeutic agent for the treatment"
symptom
achondroplasia
esophageal carcinoma
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