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Our Team
[Effect of NF-κB activation on the radiation response of esophageal cancer cells].
[esophageal carcinoma]
To
investigate
the
effect
of
NF-κB
activation
on
radiation
response
of
esophageal
carcinoma
.
The
expression
of
NF-κB
was
detected
in
pretreatment
and
posttreatment
specimens
of
patients
with
ESCC
by
immunohistochemistry
.
Electrophoretic
mobility
shift
assay
(
EMSA
)
and
Western
blot
were
used
to
detect
the
activation
of
NF-κB
in
esophageal
cancer
cell
line
KYSE
150
cells
.
SN
50
,
a
specific
NF-κB
inhibitor
,
was
applied
to
inhibit
the
activation
of
NF-κB
.
Clone
formation
test
was
used
to
detect
the
radiosensitivity
of
esophageal
cancer
cells
.
The
median
survival
time
of
patients
with
activated
and
inactivated
NF-κB
in
the
pretreatment
specimens
were
16
and
19
months
,
respectively
,
with
a
non-significant
difference
between
the
two
groups
(
P
>
0
.
05
)
.
As
to
the
patients
with
activated
and
inactivated
NF-κB
in
posttreatment
specimens
,
the
median
survival
times
were
13
and
35
months
,
respectively
,
with
a
significant
difference
(
P
<
0
.
01
)
between
them
.
Western
blot
showed
that
the
cytoplasmic
expression
of
NF-κB
was
reduced
with
increasing
radiation
dose
at
1
.
5
and
3
hours
after
radiation
treatment
.
However
,
the
expression
of
NF-κB
in
the
cell
nuclei
was
increased
under
the
same
condition
,
showing
a
trend
of
increased
nucleus
/
cytoplasm
ratio
.
The
clone
number
in
SN
50
group
was
96
.
66
,
64
.
66
,
76
.
66
and
10
.
00
under
0
,
2
,
4
and
12
Gy
irradiation
,
which
demonstrated
a
significant
difference
compared
with
the
control
groups
(
P
<
0
.
001
)
.
Our
results
show
that
activation
of
NF-κB
is
induced
by
radiotherapy
.
Activation
of
NF-κB
reduces
the
outcome
of
radiation
treatment
of
esophageal
cancer
patients
.
Diseases
Validation
Diseases presenting
"esophageal carcinoma"
symptom
esophageal adenocarcinoma
esophageal carcinoma
esophageal squamous cell carcinoma
This symptom has already been validated