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An immunofluorescent method for characterization of Barrett's esophagus cells.
[esophageal adenocarcinoma]
Esophageal
adenocarcinoma
(
EAC
)
has
an
overall
survival
rate
of
less
than
17
%
and
incidence
of
EAC
has
risen
dramatically
over
the
past
two
decades
.
One
of
the
primary
risk
factors
of
EAC
is
Barrett
's
esophagus
(
BE
)
,
a
metaplastic
change
of
the
normal
squamous
esophagus
in
response
to
chronic
heartburn
.
Despite
the
well-established
connection
between
EAC
and
BE
,
interrogation
of
the
molecular
events
,
particularly
altered
signaling
pathways
involving
progression
of
BE
to
EAC
,
are
poorly
understood
.
Much
of
this
is
due
to
the
lack
of
suitable
in
vitro
models
available
to
study
these
diseases
.
Recently
,
immortalized
BE
cell
lines
have
become
commercially
available
allowing
for
in
vitro
studies
of
BE
.
Here
,
we
present
a
method
for
immunofluorescent
staining
of
immortalized
BE
cell
lines
,
allowing
in
vitro
characterization
of
cell
signaling
and
structure
after
exposure
to
therapeutic
compounds
.
Application
of
these
techniques
will
help
develop
insight
into
the
mechanisms
involved
in
BE
to
EAC
progression
and
provide
potential
avenues
for
treatment
and
prevention
of
EAC
.
