RING finger proteins are involved in the progression of barrett esophagus to esophageal adenocarcinoma: a preliminary study.

[esophageal adenocarcinoma]

To investigate the differential expression of RING finger (RNF ) proteins in Barrett esophagus (BE ) and esophageal adenocarcinoma (EAC ).The differential expression of RNFs in normal esophagus (NE ), BE , and EAC was screened using microarray assay . Real-time quantitative polymerase chain reaction (PCR ), tissue micro-array assay , and Western blot analysis were independently performed to detect the mRNA and protein expression of screened RNFs .T he expression of nine RNFs in the BE or EAC was 2 -fold higher than those in NE . Among these proteins , the RNF 32 and RNF 121 expression in BE was 20 .3 -fold and 16 .4 -fold higher , respectively , than that in NE , and the expression of RNF 24 , RNF 130 , RNF 141 , RNF 139 , RNF 11 , RNF 14 , and RNF 159 was upregulated more than 2 -fold compared with NE . The expression of nine RNFs was not only upregulated in the EAC but was also positively related to the RNF expression in BE . The PCR results also indicated increased expression of these RNFs in BE and EAC compared to NE . Furthermore , the mRNA expression of all RNFs , except for RNF 141 in EAC , was dramatically higher than those in the BE . Similar results were also obtained from the Western blot analysis .A total of nine RNFs play critical roles in the progression of BE to EAC .

Diseases
Validation

Diseases presenting "eac compared to ne" symptom

esophageal adenocarcinoma

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