Human cord blood-derived unrestricted somatic stem cells promote wound healing and have therapeutic potential for patients with recessive dystrophic epidermolysis bullosa.

[dystrophic epidermolysis bullosa]

Human umbilical cord blood (CB )-derived unrestricted somatic stem cells (USSCs ) have previously been demonstrated to have a broad differentiation potential and regenerative beneficial effects when administered in animal models of multiple degenerative diseases . Here we demonstrated that USSCs could be induced to express genes that hallmark keratinocyte differentiation . We also demonstrated that USSCs express type VII collagen (C 7 ), a protein that is absent or defective in patients with an inherited skin disease , recessive dystrophic epidermolysis bullosa (RDEB ). In mice with full-thickness excisional wounds , a single intradermal injection of USSCs at a 1 -cm distance to the wound edge resulted in significantly accelerated wound healing . USSC-treated wounds displayed a higher density of CD 31 (+) cells , and the wounds healed with a significant increase in skin appendages . These beneficial effects were demonstrated without apparent differentiation of the injected USSCs into keratinocytes or endothelial cells . In vivo bioluminescent imaging (BLI ) revealed specific migration of USSCs modified with a luciferase reporter gene , from a distant intradermal injection site to the wound , as well as following systemic injection of USSCs . These data suggest that CB-derived USSCs could significantly contribute to wound repair and be potentially used in cell therapy for patients with RDEB .

Diseases
Validation

Diseases presenting "skin disease" symptom

child syndrome

dystrophic epidermolysis bullosa

epidermolysis bullosa simplex

harlequin ichthyosis

junctional epidermolysis bullosa

kindler syndrome

lamellar ichthyosis

omenn syndrome

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