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A random Abstract
Our Project
Our Team
Generation of 3D skin equivalents fully reconstituted from human induced pluripotent stem cells (iPSCs).
[dystrophic epidermolysis bullosa]
Recent
generation
of
patient-
specific
induced
pluripotent
stem
cells
(
PS-iPSCs
)
provides
significant
advantages
for
cell-
and
gene
-based
therapy
.
Establishment
of
iPSC-based
therapy
for
skin
diseases
requires
efficient
methodology
for
differentiating
iPSCs
into
both
keratinocytes
and
fibroblasts
,
the
major
cellular
components
of
the
skin
,
as
well
as
the
reconstruction
of
skin
structures
using
these
iPSC-derived
skin
components
.
We
previously
reported
generation
of
keratinocytes
from
human
iPSCs
for
use
in
the
treatment
of
recessive
dystrophic
epidermolysis
bullosa
(
RDEB
)
caused
by
mutations
in
the
COL
7
A
1
gene
.
Here
,
we
developed
a
protocol
for
differentiating
iPSCs
into
dermal
fibroblasts
,
which
also
produce
type
VII
collagen
and
therefore
also
have
the
potential
to
treat
RDEB
.
Moreover
,
we
generated
in
vitro
3
D
skin
equivalents
composed
exclusively
human
iPSC-derived
keratinocytes
and
fibroblasts
for
disease
models
and
regenerative
therapies
for
skin
diseases
,
first
demonstrating
that
iPSCs
can
provide
the
basis
for
modeling
a
human
organ
derived
entirely
from
two
different
types
of
iPSC-derived
cells
.
Diseases
Validation
Diseases presenting
"type vii collagen"
symptom
dystrophic epidermolysis bullosa
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