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Prognostic relevance of Fédération Nationale des Centres de Lutte Contre le Cancer grade and MDM2 amplification levels in dedifferentiated liposarcoma: a study of 50 cases.
[dedifferentiated liposarcoma]
Dedifferentiated
liposarcoma
represents
a
form
of
liposarcoma
composed
of
a
non-lipogenic
sarcoma
associated
with
well-differentiated
liposarcoma
.
The
prognostic
significance
of
histological
grading
of
the
dedifferentiated
component
remains
to
be
elucidated
due
to
vague
grading
criteria
employed
in
previous
studies
.
Molecular
markers
of
tumor
behavior
,
including
amplification
levels
of
murine
double
minute
-
2
(
MDM
2
)
and
cyclin-dependent
kinase-
4
(
CDK
4
)
genes
,
have
been
explored
in
a
limited
number
of
cases
.
Here
we
investigate
whether
'
Fédération
Nationale
des
Centres
de
Lutte
Contre
le
Cancer
'
(
FNCLCC
)
grade
and
MDM
2
gene
amplification
levels
have
prognostic
value
in
dedifferentiated
liposarcoma
in
terms
of
local
recurrence
and
disease-
specific
survival
.
Fifty
cases
were
retrieved
,
reviewed
and
FNCLCC
grade
was
scored
for
the
dedifferentiated
component
.
Testing
for
MDM
2
gene
amplification
was
performed
by
fluorescence
in
situ
hybridization
.
Amplification
was
categorized
as
high
level
(
≥
20
copies
)
and
as
low
level
(
<
20
copies
)
.
Follow-up
data
was
obtained
through
chart
review
.
Log-rank
test
and
Cox
proportional
hazard
models
were
used
to
determine
the
effect
of
grade
and
level
of
MDM
2
amplification
on
outcomes
.
Our
series
includes
50
patients
(
male
n
=
28
,
female
n
=
22
)
with
an
average
age
of
63
years
(
range
,
28
-
88
)
and
a
median
follow-up
of
28
months
(
range
,
2
-
120
)
.
Tumors
were
graded
as
grade
1
(
6
%
)
,
grade
2
(
58
%
)
,
and
grade
3
(
36
%
)
.
When
adjusted
for
age
,
sex
,
site
,
tumor
size
,
and
margin
status
,
grade
3
patients
had
a
higher
recurrence
rate
than
grades
1
and
2
(
HR
=
2
.
07
,
95
%
CI
:
1
.
24
,
7
.
62
;
P
=
0
.
015
)
.
Patients
with
high
-level
MDM
2
amplification
had
higher
recurrence
rate
on
univariate
analysis
(
P
=
0
.
028
)
,
but
not
on
multivariate
analysis
(
HR
=
1
.
69
,
95
%
CI
:
0
.
73
,
3
.
94
;
P
=
0
.
221
)
.
FNCLCC
grade
3
dedifferentiation
confers
a
worse
prognosis
in
dedifferentiated
liposarcoma
in
terms
of
local
recurrence
.
MDM
2
amplification
level
remains
a
useful
diagnostic
tool
in
dedifferentiated
liposarcoma
,
but
has
no
prognostic
value
in
terms
of
local
recurrence
.
Modern
Pathology
advance
online
publication
,
25
July
2014
;
doi
:
10
.
1038
/
modpathol
.
2014
.
88
.
Diseases
Validation
Diseases presenting
"liposarcoma in terms"
symptom
dedifferentiated liposarcoma
liposarcoma
well-differentiated liposarcoma
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